UPLC-UV法测定不同产地青蒿中青蒿素的含量  

Determination of Artemisinin in Herba Artemisia Annuae from Different Locations by UPLC-UV

作　　者：向文[1] 李琳[1] 刘吉华[1] 余伯阳[1,2] 

机构地区：[1]中国药科大学中药复方研究室 [2]中国药科大学现代中药重点实验室,江苏南京210009

出　　处：《中国野生植物资源》2012年第4期28-31,共4页Chinese Wild Plant Resources

基　　金：教育部博士学科点专项科研基金(20110096130002);江苏高校优势学科建设工程项目(PAPD)

摘　　要：目的:建立快速简便检测青蒿素的超高效液相-紫外(UPLC-UV)法,并对不同产地青蒿中青蒿素的含量进行检测。方法:色谱柱Agilent Eclipse Plus C18(2.1 mm×50 mm,1.8μm),流动相乙腈/水(45/55),流速1.0mL/min,柱温28℃,波长200 nm。结果:该方法对青蒿素的分离度较好,保留时间缩短为1.5 min。并且,整个分析过程可以在2 min内完成。线性范围0.101 17～10.117μg,进样量与峰面积线性相关,A=109.4C+6.7026,R2=0.9 993(n=9),加样回收率为99.3%(RSD=2.6%,n=6)。结论:UPLC-UV法分析时间短、样品前处理简单、精密度、稳定性、加样回收率等符合分析检测要求,对于青蒿中青蒿素的含量能进行快速准确的分析。Objective： To establish a simple, rapid and accurate method for the determination of artemisinin in Artemisia annua from different locations by Ultra Performance Liquid Chromatography - Ultraviolet （UPLC -UV）. The separation was performed on Agilent Eclipse Plus Ci8 column （2. 1 mm×50 mm, 1.8 μm）. Methods：The mobile phase consisted of acetonitrile / water （ 45 / 55 ） at the flow rate of 1.0 mL/min. The detection wavelength was set at 200 nm and column temperature was 28 ℃. Results： Comparing with the other methods, separation of artemisinin is better and retention time （ 1.5 minutes ） is shorter. Furthermore, the determination by UPLC would completed in 2 minutes. The linear rang of ar- temisinin was 0.10 117-10. 117 μg, A = 109.4 C ＋ 6.7 026, R2 = 0.9 993 （n=9） by UPLC Method. The average recovery was 99.3% （ RSD = 2.6 % ,n =6）. Conclusion： UPLC - UV is a simple, rapid, accurate and stable method for the determination of artemisinin in Artemisia annua.

关 键 词：UPLC-UV 青蒿素 含量测定 

分 类 号：R284.1[医药卫生—中药学]