RP-HPLC测定玉红生肌凝胶中羟脯氨酸的含量  

Determination of Hydroxyproline in Yuhong Shengji Gel by RP-HPLC

作　　者：高鹏[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《辽宁中医杂志》2012年第9期1819-1820,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划项目(2008BAI53B073)

摘　　要：目的:建立高效液相色谱法测定玉红生肌凝胶中羟脯氨酸含量。方法:采用Shimadzu-C18柱,以甲醇为流动相A,以醋酸-醋酸钠缓冲液(pH6.0)为流动相B为流动相进行梯度洗脱,检测波长为254nm。结果:测得羟脯氨酸线性范围0.01594～0.1434μg(r=0.9999),加样回收试验测得羟脯氨酸平均回收率为97.23%,RSD为1.20%(n=6)。结论:本法准确,专属性强。Objective ： To establish a HPLC method for determination of the content of Hydroxyproline in Yuhong Shengji Gel. Methods：Shimadzu -C18 was used as the column, methanol and sodium acetate buffer solution（pH6.0） as the mobile phase. The detection wavelength was 254nm. Results ： The Linear correlation observed was the range of 0. 01594 - 0. 1434μg （ r = 0. 9999 ）. The average recovery rate was 97.23% , RSD = 1.20% （n = 6）. Conclusion：The method is accurate and specific.

关 键 词：RP-HPLC 玉红生肌凝胶 羟脯氨酸 

分 类 号：R284[医药卫生—中药学]