枳实不同方法提取物及挥发油成分的GC-MS分析  

Analysis of Volatile Oil Components in Fructus Aurantii Immaturus Extracted with Different Methods by GC-MS

作　　者：施学骄[1,2] 韦正[1,2] 张杰红[1,2] 刘友平[1,2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《辽宁中医杂志》2012年第9期1826-1829,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：四川省教育厅自然科学重点项目(09ZA033)

摘　　要：目的:分析不同提取方法对枳实挥发油化学成分组成的影响。方法:分别采用水蒸气蒸馏法、2010年中国药典附录XD甲法、溶剂回流法、超声法提取枳实挥发油并通过GC-MS法进行成分分析,然后用归一化法计算各成分相对含量。结果:2010年中国药典附录XD甲法提取的挥发油成分丰富,被鉴定的成分占挥发油总量的70.39%。结论:不同提取方法对枳实挥发油成分的影响较大,除传统的水蒸气蒸馏法外,药典甲法方可考虑。Objective：To analyze the different extraction methods on chemical composition of volatile oil in Fructus Aurantii Immaturus. Methods：The volatile oil was extracted by steam distillation, XD A method in 2010 Chinese Pharmacopoeia Appendix, solvent reflux and ultrasonic extraction. GC - MS was used for composition analysis, Then area normalization method was used to calculate the relative content of each component. Results ： Components of volatile oil extracted by XD A method in 2010 Chinese Pharmacopoeia Appendix were rich and identified in the total composition of 70.39%. Conclusion ： Different extraction methods have important influence on chemical constituents of the volatile oil from Fruetus Aurantii Immaturus. Except for the steam distillation, the A method in Pharmacopoeia may be considered.

关 键 词：枳实 挥发油 气相色谱-质谱联用法 化学成分 

分 类 号：R284.1[医药卫生—中药学]