虾夷马粪海胆CYP51基因cDNA的克隆及其SNPs分析  

Cloning and SNPs analysis of CYP51 gene cDNA in sea urchin Strongylocentrotus intermedius

作　　者：仇雪梅[1,2] 吴领知 郭晓黎[2] 张伟杰[1] 丛玉婷[2] 常亚青[1,2] 宋坚[1,2] 刘洋 王秀利[2] 

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 [2]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《大连海洋大学学报》2012年第4期294-299,共6页Journal of Dalian Ocean University

基　　金：辽宁省自然科学基金资助项目(20102043);辽宁省"百千万人才工程"项目(2010921093)

摘　　要：采用RT-PCR、PCR-SSCP等分子生物学技术和关联分析等方法对虾夷马粪海胆Strongylocentrotusintermedius CYP51基因的cDNA序列进行了克隆和单核苷酸多态性分析。结果表明:虾夷马粪海胆的CYP51基因包括一个1 491 bp的开放阅读框,编码496个氨基酸;其开放阅读框的第161个核苷酸处存在1个单核苷酸多态性,即核苷酸发生了A→G突变。关联分析结果表明,该单核苷酸多态性与虾夷马粪海胆的体质量、性腺质量之间存在显著相关性(P<0.05)。本研究中首次克隆了虾夷马粪海胆CYP51基因的cDNA序列并进行了SNPs分析,研究结果可为虾夷马粪海胆的分子标记辅助育种提供参考。In this study,CYP51 gene was cloned and its single nucleotide polymorphisms（SNPs） was detected by reverse transcription polymerase chain reaction（RT-PCR） and polymerase chain reaction single-strand conformation polymorphism（PCR-SSCP） in sea urchin Strongylocentrotus intermedius.The results showed that the 1 536 bp sequence including a 1 491 bp open reading frame（ORF） and encoding 496 amino acids was obtained from CYP51 gene.A SNP A→G was found in the 161st nucleotide of open reading frame in CYP51 cDNA.The analysis of the SNPs showed that there was a significant association（P〈0.05） between the SNP and growth traits such as gonad weight and body weight.The CYP51 cDNA cloning and its SNPs analysis was the first time conducted in sea urchin Strongylocentrotus intermedius.The findings provide basic theory of molecular marker-assisted breeding of the sea urchin.

关 键 词：虾夷马粪海胆 CYP51基因 克隆 单核苷酸多态性 

分 类 号：S917[农业科学—水产科学]