分子标记检测矮秆基因Rht-B1b、Rht-D1b和Rht8在我国小麦中的分布  

Distribution of dwarfing genes Rht-B1b,Rht-D1b and Rht8 in Chinese bread wheat cultivars detected by molecular markers

作　　者：唐娜 李博[2] 闵红 胡银岗[3] 

机构地区：[1]陕西省食品药品检验所,西安710061 [2]第四军医大学唐都医院,西安710038 [3]陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《中国农业大学学报》2012年第4期21-26,共6页Journal of China Agricultural University

基　　金：国家"863"计划重点项目子课题(2006AA100201;2006AA100223);科技部"973"计划前期项目(2006CB708208);澳大利亚ACIAR项目(CIM/2005/111);高等学校学科创新引智计划项目(111-2-16)

摘　　要：利用分子标记检测矮秆基因在我国主要麦区的分布,有助于提高小麦产量和改良株高。本研究利用小麦矮秆基因Rht-B1b、Rht-D1b的4对特异性分子标记,BF与MR1、BF与WR1、DF与MR2、DF2与WR2,以及微卫星Xgwm261标记对我国小麦主产区小麦主栽品种中矮秆基因Rht-B1b、Rht-D1b和Rht8的分布情况进行了分子标记鉴定。结果表明:1)在鉴定的129个品种中,58份含有Rht-B1b基因,占45.0%;24份含有Rht-D1b基因,占18.6%;73份含有Rht8基因,占56.6%;35份品种含有2个矮秆基因Rht-B1b和Rht8,占27.1%;16份品种含有Rht-D1b和Rht8基因,占12.4%。本研究未检测到同时含有Rht-B1b、Rht-D1b和Rht8这3个矮秆基因的品种,以及同时含有Rht-B1b和Rht-D1b的品种;2)3个矮秆基因Rht-B1b、Rht-D1b和Rht8在各个生态区育成品种中的分布频率也不同。矮秆基因Rht-B1b和Rht8在黄淮冬麦区的分布频率较高,分别为55.4%和71.1%;Rht-D1b基因在西南冬麦区的分布频率较高,为37.5%;矮秆基因Rht8在不同的麦区都有广泛的分布,在不同的生态区具有广泛的适应性。To understanding the distribution of wheat dwarfing genes is crucial for improvement of yield and plant height. In this study, four pairs of molecular markers, BF and MR1, BF and WR1, DF and MR2, DF2 and WR2, and microsatellite Xgwm261 ,were used to identify Rht-BTb,Rht-Dlb and Rht8 dwarfing genes in the major Chinese wheat varieties. The results showed that： 1）The screening of 129 varieties revealed that 58（45.0% ） genotypes with the Rht- B1b ,24（ 18.6% ） genotypes with the Rht-D1b ,73（56.6% ） genotypes with the Rht8,35（27.1% ） genotypes with Rht- Blb ＋ Rht8 ,at and 16（12.4% ） genotypes with Rht-Dlb ＋ RhtS. None of the combination of the three dwarfing genes and Rht-B1b ＋ Rht-D1b were identified in this study. 2）the distribution frequency of the three dwarfing genes in bread varieties from different ecological regions were different. Dwarfing genes Rht-Blb and Rht8 were mostly identified from varieties from Yellow and Huai Valley Facultative Wheat Region, and the frequency were 55. 4% and 71. 1%, respectively ; The Rht-D1b gene was mainly found in Southwestern China Autumn-sown Spring Wheat Region with afrequency of 37.5% ;The Rht8 gene extensively distributed in all the ecological regions. From this study,we concluded that different dwarfing genes have different regional distribution, but the Rht8 gene extensively distributed in Chinese bread wheat varieties.

关 键 词：普通小麦 矮秆基因 Rht-B1b Rht-D1b Rht8 分子标记 微卫星 

分 类 号：S512[农业科学—作物学]