遮荫对忽地笑鳞茎中石蒜碱和加兰他敏含量的影响  

Effect of shading on contents of lycorine and galantamine in the bulb of Lycoris aurea

作　　者：全妙华[1] 佘朝文[1] 欧立军[1] 陈东明[1] 

机构地区：[1]怀化学院生命科学系/民族药用植物资源研究与利用湖南省重点实验室/湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《中国农业大学学报》2012年第4期58-61,共4页Journal of China Agricultural University

基　　金：湖南省高校创新平台开放基金项目(11K051);湖南省科技计划重点项目(2009FJ2008)

摘　　要：探讨不同光照强度条件下忽地笑鳞茎中石蒜碱和加兰他敏含量变化,为忽地笑规范化栽培提供科学依据。采用HPLC方法测定了100%自然光、50%遮荫和85%遮荫条件下忽地笑鳞茎中石蒜碱和加兰他敏含量。3种不同光强下忽地笑鳞茎中石蒜碱和加兰他敏含量均表现为:50%遮荫>85%遮荫>100%自然光,其中在50%遮荫条件下其含量均最高,与100%自然光的相比,分别提高了35.88%和20.56%,差异均达到极显著水平(P<0.01)。稳定性及回收率实验结果表明:HPLC法测定忽地笑鳞茎中石蒜碱和加兰他敏含量效果好,稳定性强,方法可靠;适度遮荫(如50%遮荫)可以提高忽地笑鳞茎中石蒜碱和加兰他敏含量。忽地笑适宜在一定遮荫环境下栽培。Changing of lycodne and galantamine contents in the bulb of Lycoris aurea under different light intensity conditions were analyzed in order to provide scientific information for cultivation of Lycoris aurea. Contents of lycorine and galantamine in the bulbs under 100% natural light,50% shading and 85% shading were determined by HPLC. The contents of lycorine and galantamine in the bulbs under the three different conditions were as following：50% shading 〉 85% shading 〉100% natural light. The contents of lycorine and galantamine under 50% shading were both the highest, and increased 35.88% and 20.56% compared with those under 100% natural light, respectively with high significant level （P〈0.01）. The results determined by HPLC were highly repeatable, indicating that the method used in this study was reliable. Proper shading, such as 50% shading, increased contents of lycorine and galantamine in the bulb of Lycoris aurea. Thus Lycoris aurea was suitable for cultivation under moderate shading.

关 键 词：忽地笑 遮荫 HPLC 石蒜碱 加兰他敏 

分 类 号：R282.2[医药卫生—中药学]