Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles  

作　　者：FU Yu-qin LI Lu-hua WANG Pi-wu QU Jing FU Yong-ping WANG Hui SUN Jing-ran LÜ Chang-li 

机构地区：[1]College of Life Science,Jilin Agricultural University,Changchun 130018,P.R.China [2]Faculty of Agronomy,Jilin Agricultural University,Changchun 130018,P.R.China [3]Faculty of Chemistry,Northeast Normal University,Changchun 130024,P.R.China

出　　处：《Chemical Research in Chinese Universities》2012年第4期672-676,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.21074019);the China Postdoctoral Science Foundation Funded Project(No.200904501024);the Natural Science Foundation of Jilin Province,China(No.20101539);the Jilin Province Science and Technology Development Project,China(No.20090155)

摘　　要：The development of nanotechnology provides a new method for genetic engineering.However,the nanoparticles as gene carriers have been mainly used in the mammalian cells so far.We observed that ZnS nanoparticles modified with positively charged poly-L-lysine（PLL） successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted method.Polymerase chain reaction（PCR） detection,Southern blot analysis and GUS histochemical staining were carried out for the regenerated plants.The stable genetic modified plants mediated by ZnS nanoparticles can be obtained.This article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.The development of nanotechnology provides a new method for genetic engineering.However,the nanoparticles as gene carriers have been mainly used in the mammalian cells so far.We observed that ZnS nanoparticles modified with positively charged poly-L-lysine（PLL） successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted method.Polymerase chain reaction（PCR） detection,Southern blot analysis and GUS histochemical staining were carried out for the regenerated plants.The stable genetic modified plants mediated by ZnS nanoparticles can be obtained.This article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.

关 键 词：ZnS nanoparticle Gene carrier TRANSFORMATION TOBACCO Stable expression 

分 类 号：Q782[生物学—分子生物学] TN304.22[电子电信—物理电子学]