小檗碱对甲亢性腹泻大鼠结肠及血浆中神经肽Y含量的影响  

Investigate the Effect of Berberine on Neuropeptide Y in Rats Colon Tissue and Plasma of Thyroid Hormone-Induced Diarrheic

作　　者：霍莹莹[1] 李芸兰[1] 成志锋[1] 李波[1] 王明丽[1] 

机构地区：[1]哈尔滨医科大学附属第四医院内分泌科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2012年第24期4620-4623,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨小檗碱对甲状腺功能亢进(甲亢)性腹泻大鼠结肠及血浆中神经肽Y的影响,进而研究小檗碱治疗甲亢性腹泻的机制。方法:制备甲亢性腹泻大鼠模型。应用小檗碱对其干预,采用免疫组化方法测定甲亢性腹泻组、小檗碱治疗剂量组、健康组大鼠结肠神经肽Y的定位,采用酶联免疫方法检测各组大鼠血浆中神经肽Y含量的变化。结果:在结肠组织中,甲亢性腹泻组与健康对照组比较NPY表达明显增多,经小檗碱治疗后NPY表达较甲亢性腹泻组表达减少,接近健康对照组的表达。在血浆中,NPY的表达在甲亢性腹泻组与健康对照组中增多,经小檗碱治疗后NPY表达较甲亢性腹泻组表达减少,接近健康对照组的表达。结论:甲亢性腹泻大鼠结肠及血浆中NPY的表达明显增多,小檗碱治疗后NPY的表达又减少,接近健康对照组。NPY可能参与甲亢性腹泻的发生机制.Objective： To investigate the effect of berberine on Neuropeptide Y （NPY） in colon and plasma of thyroid hormone-induced diarrheic （hyperthyroid diarrhea,HD） rats and to study the mechanism of berberine in the treatment of hyperthyroidisnl. Methods： The model of thyroid homlone-induced diarrheic rats were prepared and interfered by berberine. Immunohistochemistry was used to detect the location of the Neuropeptide Y in colon of hyperthyroid diarrhea group, dose group of berberinc and healthy group. ELISA was used to detect the content ofNeuropeptide Y in plasma of each group. Results： NPY expression in colon tissue, hyperthyroid diarrhea group increased compared with that in the healthy group. NPY expression in berberine treatment group decreased compared with that in the hyperthyroid diarrhea group, closed to the expression of the healthy control group. In plasma, NPY expression in tile hyperthyroid group and diarrhea in healthy control group increased. NPY expression in berberine treatment group compared with that in hyperthyroid diarrhea group decreased, and which was close to the expression of the healthy control group. Conclusion： NPY expression increased significantly in rats colon tissue and plasma of thyroid hormone-induced diarrheic. NPY expression reduced by berberine treatment, closed to the healthy control group. NPY may be involved in the mechanism of diarrhea in hyperthyroidism.

关 键 词：甲状腺功能亢进 腹泻 小檗碱 神经肽Y 

分 类 号：Q95-3[生物学—动物学] R581.1[医药卫生—内分泌]