探讨尿视黄醇结合蛋白对高血压早期肾损害的作用研究  

Explore Urine Retinol Combined with Protein to High Blood Pressure Early Renal Damage Role Research

作　　者：刘红[1] 崔建英[1] 岳华[1] 陆晨[1] 刘颖[1] 赵红娟[1] 

机构地区：[1]新疆维吾尔自治区人民医院肾脏病研究室,新疆乌鲁木齐830001

出　　处：《现代生物医学进展》2012年第24期4685-4687,4717,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨尿视黄醇结合蛋白(RBP)、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、尿胱蛋白酶抑制剂C(Cystatin C)的测定对原发性高血压早期肾损害诊断的敏感性及临床意义。方法:选择2011年4月～2011年11月在新疆自治区人民医院肾病科住院的原发性高血压患者69例作为观察组,另设健康体检者30例为对照组。尿RBP、Cystatin C测定采用酶联免疫法(ELISA)检测,尿NAG测定采用对硝基苯酚(PNP)比色法检测。结果:观察组中尿RBP、NAG、Cystatin C的测定含量明显高于正常对照组(P<0.001),通过ROC曲线、诊断试验结果显示:尿RBP的曲线下面积为0.962,95%可信区间为0.921～0.983,较尿NAG、Cystatin C更具有敏感性(P<0.001)。结论:检测尿RBP有助于高血压肾损害的早期监测,对预防高血压性肾病的发生、发展具有重要的临床价值。Objective： The sensitive index and clinical significance in early renal injury caused by primary hypertension was inves- tigate by detecting Urine retinol combined with protein（RBP）, N-acetyl-β-D-amino grape glycosidase （NAG）, urine micro proteinase in- hibitor C （Cystatin C）. Methods： 69 patients with primary hypertension were enrolled who were in-patients, dated from April 2011 to November 2011; 30 subjects of healthy people for the outpatient were selected as control group. The urine RBP and urine Cystatin C were respectively determined by enzyme linked immunosorbent assay （ELISA）. Urine NAG was measured by p-nitrophenol （PNP） col- orimetric assay. Results： In case group urine RBP, NAG and the Cystatin C determination content were higher that in the normal control group, the differences were statistically significant （P〈0.001）.Through the ROC curve and diagnostic test, the area under the curve of Urine RBP were 0.962, the 95% confidence interval were 0.921 ~0.983（P〈0.001）. Conclusions： The urine RBP could be helpful in early detection of hypertensive renel injury, and there was important clinical value in the prevention of hypertensive renel injury. .

关 键 词：尿视黄醇结合蛋白 尿N-乙酰-Β-D-氨基葡萄糖苷酶 尿胱蛋白酶抑制剂C 高血压早期肾损害 

分 类 号：R544.1[医药卫生—心血管疾病]