IGF-1在不同鸡与鹌鹑杂交禽胚胎肌肉发育过程中的表达规律与差异  

Developmental Expressional Profiles of IGF-1 during Embryonic Myogenesis in Two Strains of Hybrid of Chicken - Quail

作　　者：梁耀伟[1] 赵宗胜[1] 陈丹盈[1] 赵松华[1] 张慧[1] 班谦[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《新疆农业科学》2012年第9期1717-1722,共6页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金(30660125)

摘　　要：【目的】研究IGF-1基因在蛋用型种鸡(♂)×鹌鹑(♀)和肉用型种鸡(♂)×鹌鹑(♀)两种不同杂交禽胚胎肌肉发育过程中的表达规律,比较该基因在两种不同杂交禽中的差异表达,探讨IGF-1基因在肌肉发育网络调控中的作用。【方法】通过人工受精的方法,蛋用型种鸡(♂)×鹌鹑(♀)获得蛋杂交禽蛋、肉用型种鸡(♂)×鹌鹑(♀)获得肉杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批人孵,采集第7～17 d活胚胸肌组织,应用实时荧光定量PCR技术检测IGF-1基因在四个物种中每个时间点mRNA的相对表达量。【结果】IGF-1基因在胚胎肌肉发育的第7～17 d均有表达,在第10 d达到第一次峰值,鸡和鹌鹑在第16 d达到第二次峰值,蛋杂交禽和肉杂交禽在第15 d达到峰值。【结论】IGF-1可能在胚胎肌肉的生长发育过程中起调控作用;IGF-1 mRNA表达规律与成肌细胞增殖和肌管融合规律一致,可能参与调控成肌细胞的增殖和肌管的融合。[ Objective ] The purpose of this study was to analyze IGF - 1 mRNA expression profiles and differences during embryonic myogenesis in two strains of hybrid of chicken-quail, and evaluate the potential relationships between IGF - 1 expression and myogenesis. [ Method ] Two strains of cross - bred eggs of quail （ ♀ ） were acquired by artificial insemination by chicken（ /5 ） - semen, with the eggs of chicken, quail and hybrid in one batch hatched according to the standard condition of chicken, and then the breast muscle tissues from day 7 to day 17 were collected. The expression of IGF - 1 mRNA in different species every day was revealed by realtime quantitative PCR. [Result]The expression of IGF- 1 mRNA was detected from day 7 to day 17. The first peak time of IGF - 1 expression was noticed at day 10 in four species, the second peak time was noticed at day 16 in Chicken and Quail, and at day 15 in Meat - Hybrid and Egg - Hybrid. [ Conclusion ] IGF - 1 maybe plays a key role in the process of myogenesis. The expression profile of IGF - 1 was in accord with the profile of myoblasts proliferation and muscle tube fusion, and it maybe plays a key role in the process of myoblasts proliferation and muscle tube fusion.

关 键 词：鸡 鹌鹑 杂交禽 肌肉发育 IGF-1基因 实时荧光定量PCR 

分 类 号：S814.8[农业科学—畜牧学]