FSHβ基因PCR-SSCP多态性及其与多浪羊高繁殖力的关系研究  

PCR - SSCP Polymorphism of FSHβ Gene and Its Relationship with Prolificacy of Duolang Sheep

作　　者：阿布来提·苏来曼[1] 买买提伊明·巴拉提[1] 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2012年第9期1727-1733,共7页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30760169)

摘　　要：【目的】研究促卵泡素β亚基(follicle-stimulating hormoneβ,FSHβ)基因在多浪羊群体中的单核苷酸多态性,并检测该基因对多浪羊繁殖力的影响。【方法】采用PCR-SSCP技术,采集产双羔及以上的多胎多浪羊血样310份,研究FSHβ基因在多浪羊群体中的多态性,同时检测基因型频率以及等位基因频率。【结果】FSHβ基因在多浪羊群体中检测到AA、AB和BB三种基因型;基因型频率分别为0.36、0.6和0.04。多浪羊A和B等位基因频率为0.66和0.34。BB型与AA型相比有6处核苷酸发生了突变,该突变导致5个氨基酸的改变。BB基因型多浪羊产羔数分别比AB和AA基因型多1.107 1只(P<0.01)和1.017只(P<0.01),AB与AA基因型之间的产羔数差异不显著(P>0.05)。【结论】FSHβ基因首次在新疆多浪羊群体中发现了BB基因型,绵羊FSHβ基因座位可能与多浪羊高繁殖力相关。[ Objective ] To research the single nucleotide polymorphism of FSHβgene in Duolang Sheep, and detect the impact of FSHβgene to the prolificacy in the sheep. [ Method ] 308 Duolang sheep were studied as experimental material and the FSHβgene was studied as a candidate gene for the prolificacy in Duolang Sheep by using PCR - SSCP ; at the same time the genotypes and genotype frequency were found out. [Result] FSHβhad a polymorphism in Duolang Sheep, the FSHβ gene had three genotypes in Duolang sheep： Wild type AA, Heterozygous mutation AB and Homozygous mutation BB; the genotype frequency was： 0. 36, 0.6 and 0.04 respectively, and the gene frequency of B allele and A allele were ： 0.34 and 0.66. The sequencing results revealed that six single nucleotide mutations were found in FSHβgene in the BB genotype in comparison to the AA genotype, and also these mutations did cause 5 amino acid changes. The ewes with genotype BB had 1.017 （P 〈0.05） and 1. 1071 （P 〈0.05） lambs more than those with genotype AA and AB in Duolang Sheep, respectively. [ Conclusion ] For the first time, BB genotype in FSHβ gene was found in Duolang Sheep populations in Xinjiang. These preliminary results indicated that the Sheep FSHβgene loci may be related to the high fecundity of Duoalng Sheep.

关 键 词：多浪羊 高繁殖力 促卵泡素β亚基基因 PCR—SSCP 

分 类 号：S826[农业科学—畜牧学] S188[农业科学—畜牧兽医]