医用高氮无镍奥氏体不锈钢的制备与生物相容性研究  

Evaluation on the blood compatibility and cell compatibility of medical high nitrogen nickel-free stainless steel in vitro

作　　者：王宏刚[1] 葛淑萍[1] 邹兴政[2] 尹铁英[1] 兰华林[1] 王英[1] 李梦华[1] 罗来龙[3] 王贵学[1] 

机构地区：[1]重庆大学生物流变科学与技术教育部重点实验室,重庆400044 [2]重庆材料研究院,重庆400707 [3]天津市东丽区东丽医院,天津300300

出　　处：《功能材料》2012年第18期2483-2487,共5页Journal of Functional Materials

基　　金：国家科技支撑计划资助项目(2012BAI18B02);国家重点基础研究发展计划(973计划)资助项目(2012CB619101);国家自然科学基金资助项目(30901977);重庆市科技计划攻关资助项目(CSTC 2010AB5116)

摘　　要：主要探讨不同含氮量的无镍奥氏体不锈钢材料对血管内皮细胞及血小板粘附的影响。通过溶血率和血小板粘附试验考察不同含氮量的无镍奥氏体不锈钢材料的血液相容性;通过MTT试验和细胞粘附试验考察不同含氮量的无镍奥氏体不锈钢材料的细胞相容性。结果表明不同含氮量的无镍奥氏体不锈钢材料的溶血率都低于国家标准的5%,对血小板粘附的影响不显著;粘附在高氮无镍奥氏体不锈钢材料表面的血管内皮细胞数量均多于钛合金材料,且细胞生长状态良好;细胞毒性试验表明,不同含氮量的高氮无镍奥氏体不锈钢材料和对照组钛合金材料对血管内皮细胞没有产生明显的毒副作用。Effects of high nitrogen nickel-free stainless steel on the adhesion of human umbilical vein endothelial cells（HUVECs） and blood platelets were investigated.The blood platelets adhesion test and blood hemolysis rate were used to evaluate the blood compatibility of high nitrogen nickel-free stainless steel.Human umbilical vein endothelial cells（HUVECs） were cultured in vitro,using MTT cytotoxicity test,cell adhesion test,to evaluate the cell compatibility of high nitrogen nickel-free stainless steel.Results showed that the hemolysis rate of high nitrogen nickel-free stainless steel was well below the accepted threshold value of 5%,but the effect on platelet adhesion was not obvious;the number of HUVECs adhesion on surface of high nitrogen nickel-free stainless steel were significantly more than that on the surface of titanium,and the cells in the surface showed a good growth situation.MTT test showed that both the high nitrogen nickel-free stainless steel and titanium alloy had no obvious effect on the HUVECs.

关 键 词：高氮无镍奥氏体不锈钢 细胞毒性 溶血率 细胞粘附 血小板粘附 

分 类 号：R318.08[医药卫生—生物医学工程]