铜绿假单胞菌lasI/lasR群体感应系统与生物膜形成相关基因表达调控的研究  

Study on relative expression regulation of Pseudomonas aeruginosa lasI/lasR quorum-sensing system and biofilm formation

作　　者：李琬琛[1,2] 魏殿军[2] 李立艳[2] 宋林[1] 刘双庆[1,2] 

机构地区：[1]天津医科大学研究生院,天津300700 [2]天津医科大学第二医院检验科,天津300000

出　　处：《中华医院感染学杂志》2012年第17期3685-3687,共3页Chinese Journal of Nosocomiology

基　　金：天津市教委高等学校科技发展基金;天津市卫生局科技基金

摘　　要：目的了解铜绿假单胞菌(PAE)生物膜形成过程中多糖合成相关基因,lasI/lasR群体感应系统及鼠李糖脂转移酶合成相关基因rhlA在生物膜形成过程中的表达,探讨其在PAE生物膜形成中的关系及调控作用。方法分别收集非黏液型铜绿假单胞菌PAO1的浮游菌及生物被膜菌,用实时荧光定量RT-PCR的方法,对基因的表达进行相对定量分析。结果 pslA1d的浮游菌表达量略高于6d生物膜菌外,algD、pelA、LasI、LasR、RhlA生物被膜菌的mRNA相对表达量均高于浮游菌,并且pslA、pelA、RhlA的mRNA相对表达量趋势一致,均是培养1d生物膜菌表达最高。结论 algD、pslA、pelA、LasI、LasR、RhlA的表达与铜绿假单胞菌生物膜形成密切相关,在生物膜形成中具有重要作用,同时群体感应系统很可能参与pslA、pelA、RhlA的正向转录调节。OBJECTIVE To study the relative expression of polysaccharide biosynthesis genes,lasI/lasR quorum-sensing system and rhamnolipid transferase biosynthesis gene rhlA during the formation of Pseudomonas aeruginosa biofilm,and analyze the correlation among them.METHODS Non-mucoid P.aeruginosa PAO1 planktonic bacteria and biofilm bacteria were collected,real-time fluorescence quantitative RT-PCR method was employed for the analysis of relative quantitative of the gene expression.RESULTS In addition to relative expression of pslA gene of one day planktonic bacteria was slightly higher than its six days of biofilm bacteria,algD,pelA,LasI,LasR,RhlA biofilm bacteria mRNA relative expression were all higher than planktonic bacteria,and pslA,pelA,RhlA of mRNA expression of 1d biofilm bacteria was consistent,expressed the highest.CONCLUSION The expression of algD,pslA,pelA,LasI,LasR,and RhlA is closely related to P.aeruginosa biofilm formation and plays an important role in the biofilm formation,while the quorum-sensing system may involve the positive transcription regulation of pslA,pelA,and RhlA.

关 键 词：铜绿假单胞菌 生物膜 多糖 群体感应系统 鼠李糖脂转移酶 

分 类 号：R378.991[医药卫生—病原生物学]