多药耐药肺炎克雷伯菌氨基糖苷类修饰酶与16S rRNA甲基化酶基因研究  

Investigation of aminoglycoside modifying enzyme genes and 16SrRNA methylase genes in multidrug-resistant Klebsiella pneumoniae

作　　者：朱健铭 肖丹宇[2] 姜如金[1] 吴康乐[1] 

机构地区：[1]杭州市余杭区中医院检验科,浙江杭州311106 [2]杭州市余杭区中医院普外科,浙江杭州311106

出　　处：《中华医院感染学杂志》2012年第17期3690-3693,共4页Chinese Journal of Nosocomiology

基　　金：杭州市科技发展计划项目(20110833B48);杭州市卫生科技计划项目(2010B078);杭州市余杭区重大科技项目(2010001)

摘　　要：目的研究多药耐药肺炎克雷伯菌(MDRKP)氨基糖苷类药物耐药的遗传学背景。方法收集2008年8月-2010年5月6所医院共47株肺炎克雷伯菌,采用聚合酶链反应(PCR)的方法分析15种氨基糖苷类修饰酶和6种16S rRNA甲基化酶基因。结果该组肺炎克雷伯菌共检出4种氨基糖苷类修饰酶和1种16S rRNA甲基化酶基因,可分为16种阳性检出模式,并发现两株菌携带aac(6′)-Ⅰb基因新亚型[aac(6′)-Ⅰb-hz,美国GenBank登录号:JF901756];其他16种基因未检出。结论携带氨基糖苷类修饰酶和16S rRNA甲基化酶基因,是该组肺炎克雷伯菌对氨基糖苷类药物耐药的主要原因,在多药耐药肺炎克雷伯菌中发现aac(6′)-Ⅰb新亚型[aac(6′)-Ⅰb-hz]为国内外首次报道。OBJECTIVE To investigate genetic background of drug resistance of multidrug-resistant Klebsiella pneumoniae to aminoglycosides.METHODS From Aug 2008 to May 2010,a total of 47 strains of multidrug-resistant K.pneumoniae were collected from 6 hospitals,then,15 kinds of aminoglycoside modifying enzyme genes and 6 kinds of 16SrRNA methylase genes were analyzed by PCR.RESULTS Four kinds of aminoglycoside modifying enzyme genes and one kind of 16SrRNA methylase gene were detected,which could be classsified to 16 kinds of positive modes.Furthermore,new subtype of aac（6′）-Ⅰb gene（aac（6′）-Ⅰb-hz,GenBank：JF901756） were detected in 2 strains;however,the rest of 16 kinds of genes couldn’t be detected.CONCLUSION Aminoglycoside modifying enzyme genes and 16SrRNA methylase genes play a key role in drug resistance of K.pneumoniae to aminoglycosides.It′s the first report that the new subtype of aac（6′）-Ⅰb gene（aac（6′）-Ⅰb-hz） is detected in multidrug-resistant K.pneumoniae.

关 键 词：肺炎克雷伯菌 氨基糖苷类修饰酶基因 16S rRNA甲基化酶基因 

分 类 号：R378.996[医药卫生—病原生物学]