苦参碱对HepG2细胞DLK1基因表达及细胞生长的影响  

Effects of matrine on the growth and expression of DLK1 in HepG2 cells

作　　者：罗耀玲[1] 黄铀新[1] 刘瑶[1] 

机构地区：[1]赣南医学院第一附属医院,江西省赣州市341000

出　　处：《实用医学杂志》2012年第17期2823-2825,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81000908)

摘　　要：目的:探讨苦参碱能否重新诱导HepG2细胞DLK1基因被印记及对细胞生长的影响。方法:RT-PCR检测不同浓度苦参碱处理HepG2细胞后DLK1基因表达水平变化;MTT、transwell和流式细胞术检测苦参碱作用后HepG2细胞的增殖、凋亡及侵袭力的变化。结果:HepG2细胞经苦参碱处理后,DLK1基因的mRNA表达量降低,细胞的增殖能力、侵袭能力均降低,细胞抑制在G1期。结论:苦参碱能有效地抑制DLK1基因的表达,且能抑制肿瘤细胞HepG2生长、增殖和侵袭能力。Objective To investingate the effects of matrine on the growth and expression of DLK1 in human hepatocellular carcinoma cell line HepG2. Methods HepG2 cells were treated with different concentrations of matrine, RT-PCR was used for the detection of DLK1 gene expression level; MTr, transwell and flow cytometry were used for the detections of HepG2 cell growth, invasiveness and apoptosis. Results After treated with matrine, HepG2 cells DLK1 gene mRNA expression, cell growth rate and invasive ability decreased. Conclusion Matrine can effectively inhibit DLK1 gene expression, cell growth, proliferation and invasion of HepG2.

关 键 词：苦参碱 DLK1基因 HEPG2细胞 

分 类 号：R285.5[医药卫生—中药学]