颈总动脉损伤大鼠血管蛋白激酶C与核因子-κB的表达及辛伐他汀对其的影响  

The expression of PKC and NF-κB in vessel in rats with carotid artery injury and effect of simvastatin

作　　者：郭勇[1] 王永玲[1] 侯慧芳[1] 刘国庆[1] 黄丽君[1] 孙银平[1] 

机构地区：[1]新乡医学院病理生理学教研室,453003

出　　处：《实用医学杂志》2012年第17期2850-2852,共3页The Journal of Practical Medicine

基　　金：河南省教育厅自然科学研究计划项目(编号:2008A310012);河南省科技厅科技攻关课题基金项目资助(编号:0424410129)

摘　　要：目的:探讨蛋白激酶C(PKC)与核因子-κB(NF-κB)在血管损伤后狭窄中的作用及辛伐他汀对其的影响。方法:建立颈总动脉损伤动物模型:雄性SD大鼠48只,分为假手术组、手术组、低剂量和高剂量辛伐他汀组,低、高剂量辛伐他汀组术前3d分别用5mg/(kg·d)和10mg/(kg·d)辛伐他汀灌胃,术后14d处死。HE染色观察血管形态学的变化;Westernblot检测血管PKCα与NF-κB蛋白的表达。结果:与假手术组比较,手术组血管平滑肌细胞(VSMC)增殖显著,管腔严重狭窄;与手术组相比,辛伐他汀处理后VSMC排列趋于规整,管腔狭窄程度较轻。与假手术组比较,手术组PKCα与NF-κB蛋白差异显著(P<0.01);与手术组比较,高剂量组PKCα与NF-κB蛋白表达显著降低(P<0.01),低剂量组的表达差异无统计学意义(P>0.05);高剂量组与低剂量组有明显差异(P<0.05)。结论:PKCα与NF-κB参与血管损伤后狭窄的形成,辛伐他汀具有抗血管狭窄作用,其机制可能与下调PKCα与NF-κB表达有关。Objective To explore the effect of simvastatin on the expression of PKCα and NF-κB in vessel after carotid artery injury in rats. Methods Animal model of carotid artery injury was established, the rats were randomly divided into：sham group, operation group, low doses group and high doses simvastatin groups, the rats of low and high doses groups were respectively lavaged with 5 mg/（kg.d） and 10 mg/（kg.d） simvastatin, all used animals were dead after 14 days. The changes of tunica media vasornm was observed by HE staining; the expression changes of PKCA and NF-κB proteins were detected by Western blot. Results Compared with sham group,the VSMC was obviously proliferated and the lumen of the carotid artery became stenosis in operation group; The proliferation of VSMC was regularly arrangement and the stenosis degree became slight by simvastatin. Compared with the sham group, the expression of PKCα and NF-κB was significantly increased in operation group （P 〈 0.01 ） ; Compared with operation group, the expression of PKCα and NF-κB Was significantly decreased in high doses group （P 〈 0.01 ）, while it was unconspicuous in low doses group（P 〉 0.05）, there was significant difference between low doses group and high doses simvastatin group （P 〈 0.05）. Conclusions PKCoL and NF-κB involved in the formation of stenosis after vascular injury. Simvastatin might inhibit the expression of PKCα and NF-κB to suppress VSMC proliferation and reduce the role of vascular stenosis.

关 键 词：颈总动脉 辛伐他汀 血管 狭窄 蛋白激酶C 核因子-ΚB 

分 类 号：R543.4[医药卫生—心血管疾病]