连接酶链反应检测沙眼衣原体的准确性Meta分析  

The Accuracy for Chlamydia Trachomatis Test by Ligase Chain Reaction:A Meta-Analysis

作　　者：葛龙[1] 陈婵[2] 梁莉[1] 安妮[1] 王小琴[1] 田金徽[1] 

机构地区：[1]兰州大学循证医学中心,基础医学院,兰州730000 [2]兰州大学第二临床医学院,兰州730000

出　　处：《循证医学》2012年第4期217-223,共7页The Journal of Evidence-Based Medicine

基　　金：2011年度兰州大学中央高校基本科研业务费专项基金资助项目(lzujbky-2011-133)

摘　　要：目的评价连接酶链反应技术与培养法诊断沙眼衣原体的准确性。方法计算机检索PubMed、EMBASE、Cochrane图书馆、中国生物医学文献数据库、中国期刊全文数据库和万方数据库等,收集连接酶链反应诊断沙眼衣原体的试验研究,依据QUADAS质量评价标准评价纳入研究的质量,采用MetaAnalyst软件进行Meta分析。结果共纳入22个研究,共16073例受试者。Meta分析结果显示:与诊断沙眼衣原体感染的"金标准"细胞培养相比,连接酶链反应诊断沙眼衣原体感染的合并敏感度、特异度、阳性似然比、阴性似然比、诊断比值比和综合受试者工作特征曲线下面积分别为0.934(95%可信区间0.891～0.961)、0.983(95%可信区间0.972～0.990)、52.88(95%可信区间32.297～86.583)、0.069(95%可信区间0.043～0.110),1234.549(95%可信区间545.900～2791.925)和0.986。结论连接酶链反应可作为确诊沙眼衣原体感染的一项新技术,在检测沙眼衣原体感染中有着重要意义。Objective To evaluate the accuracy of ligase chain reaction and culture for Chlamydia trachomatis test. Methods Systematic and comprehensive literatures were searched in PubMed, EMBASE, The Coehrane Library, Chinese Biomedical Literature Database, China National Knowledge Infrastructure and Wanfang database, et al. The studies of ligase chain reaction diagnosis for Chlamydia traehomatis were included. The quality assessment of diagnostic accuracy studies （QUADAS） items were used to assess the quality of the included studies. The MetaAnalyst software was used to analyze the data. Results A total of 22 studies involving 16 073 participants were included. The results of meta-analysis showed that compared with the cell culture, which used considered as gold standard, the ligase chain reaction indicating a higher level of overall accuracy, the summary sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, that the area under the summary receiver operating characteristic （SROC） curve were 0.934（95%CI 0.891-0.961）, 0.983（95%C1 0.972-0.990）, 52.88（95%CI 32.297-86.583）, 0.069（95%CI 0.043-0.110）, 1 234.549（95%CI 545.900-2 791.925） and 0.986, respectively. Conclusion The ligase chain could use as a new technology for Chlamydia traehomatis infection diagnosed, and may be of benefit for testing Chlamydia trachomatis infection.

关 键 词：沙眼衣原体 连接酶链反应 准确性 META分析 

分 类 号：R440[医药卫生—诊断学]