猪TLR4基因2个突变位点的PCR-SSCP分析  

The analysis of the two mutation sites in the TLR4 genes in pig by PCR-SSCP

作　　者：陈月婵[1] 翟春媛[1] 刘娣 杨秀芹[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《黑龙江畜牧兽医》2012年第9期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31072007);中国博士后科学基金项目(200902426)

摘　　要：为了研究Toll样受体4(TLR4)的多态性与动物对相关病原的免疫力的相关性,试验选择北京黑猪、长白猪、野猪、杜洛克猪、大白猪、民猪6个中外猪种,利用PCR-SSCP和克隆测序方法对TLR4基因第3外显子1 824位点的G/A和3'UTR 208位点的T/C多态进行群体遗传学分析。结果表明:1 824位点北京黑猪与野猪、民猪、杜洛克猪、长白猪和大白猪的基因型分布存在显著差异(P<0.05);3'UTR 208位点基因型分布在猪种间存在极显著差异(P<0.01)。To study the Toll - like receptor 4 （TLR4） polymorphism and the correlation of immunity for animals to the relevant pathogen, Beijing black pig, Landrace, wild boar, Duroc, Large White, Chinese and foreign citizens of pig breeds were selected as experimental material, and the mutations of TLR4 gene 3th exon 1 824 loci of the G / A and 3＇UTR 208 loci of the T / C polymorphism were analyzed in population genetics by PCR - SSCP. The results showed that the genotype frequencies for Beijing black pig and wild boar, Min pig, Duroc, Landrace and Large White were significantly different （ P 〈 0.05 ） at 1 824 loci ; 3＇UTR 208 loci genotype frequencies between multiple pigs were significant difference （P〈0.01）.

关 键 词：猪 Toll样受体4(TLR4) PCR单链构象多态性(PCR—SSCP) 单核苷酸多态性(SNP) 

分 类 号：S813.3[农业科学—畜牧学]