一副蒙药复方对急性冷应激小鼠非特异性免疫水平的影响  

Effect of one compound Mongolian medicine on the level of non-specific immunity in mice under acute cold stress

作　　者：姜艳[1] 王纯洁[1] 敖日格乐[1] 马燕[1] 裴大为[1] 冉金宝[1] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018

出　　处：《黑龙江畜牧兽医》2012年第9期119-121,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31060318);内蒙古自然科学基金项目(2009ms0401);内蒙古人才基金项目

摘　　要：为了研究抗冷应激蒙药复方对急性冷应激小鼠非特异性免疫的影响,试验将昆明系小鼠随机分为冷应激组、阳性对照组、蒙药高剂量组、蒙药低剂量组,在6℃条件下冷应激0 h、2 h、24 h后从每组随机取8只小鼠称重,进行腹腔吞噬试验及脾脏自然杀伤(NK)细胞活性试验,并取胸腺、脾脏称重。结果表明:冷应激2 h、24 h,蒙药高剂量组小鼠胸腺指数极显著高于冷应激组(P<0.01);冷应激24 h,蒙药高、低剂量组小鼠脾脏指数略高于冷应激组(P>0.05);蒙药高、低剂量组小鼠吞噬指数和吞噬百分率在2,24小时均显著或极显著高于冷应激组(P<0.05或P<0.01);蒙药高、低剂量组NK细胞活性在2,24小时时均显著或极显著高于冷应激组(P<0.05或P<0.01)。说明蒙药复方可以提高6℃条件下急性冷应激小鼠的非特异性免疫水平。To study the effect of one compound Mongolian medicine on non - specific immunity in mice under cold stress, Kunming mice were randomly divided into 4 groups, including the groups for cold stress, positive control, ,high and low dose of Mongolian medicine. The Mice from four groups were removed into biochemical incubator at 6 ℃, then killed at the time of 0 h, 2 h, 24 h. Eight mice were randomly selected from each group, and weighed to detect the non - specific immunity by the tests for peritoneal macrophage phagecytosis of chicken erythrocytes and NK cell activity, weighing the spleen and thymus. The result showed that the thymus indexes of the mice in the high - dose Mongolian medicine group were significantly higher than those of in the cold stress group at the time of 2 h, 24 h （ P 〈 0.01 ）, the spleen indexes of the mice in the high and low dose Mongolian medicine group were slightly higher than those of in the cold stress group at the time of 24 h （ P 〉 0.05 ） ; the phag- ocytic indexes and the phagocytic percentages of the mice in the high and low dose Mongolian medicine group were higher or significantly higher than those of in the cold stress group at the time of 2 h, 24 h（ P 〈 0.05 or P 〈 0.01 ） ;NK cell activity of the mice in the high and low dose Mongolian medicine group were higher or significantly higher than those of in the cold stress group at the time of 2 h, 24 h （ P 〈 0.05 or P 〈 0.01 ）. The results demonstrates that Mongolian compound medicine can improve the level of non - specific immunity at 6℃.

关 键 词：蒙药复方 冷应激 小鼠 非特异性免疫 

分 类 号：S852.4[农业科学—基础兽医学]