动物源肠杆菌分离株质粒介导的喹诺酮类耐药基因的检测  

Detection of plasmid-mediated quinolone resistance genes in isolates of Enterobacteriaceae from animals

作　　者：张悦[1] 王杨[1] 张美君[1] 刘雅红[1] 

机构地区：[1]华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州510642

出　　处：《中国兽医杂志》2012年第8期15-17,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金-广东省政府自然科学基金联合基金重点项目(U0631006);广东省自然科学基金创新团队项目(5200638)

摘　　要：为了解近年广东地区肠杆菌科质粒介导喹诺酮类耐药基因(PMQR)的流行情况,对广东地区猪、禽养殖场2007～2009年分离的407株肠杆菌进行PMQR基因的检测,采用琼脂平皿二倍稀释法对所有菌株进行15种抗菌药物的敏感性试验。结果显示,qnrA、qnrB、qnrS、qnrD、qepA及aac(6′)-Ib-cr的检出率分别为0.98%、4.91%、16.22%、1.72%、0.25%、5.41%,qnrC没有检测出,有27(6.63%)株同时携带两种或两种以上PMQR基因。近年广东地区动物源肠杆菌的PMQR基因流行存在上升趋势,耐药性存在严重,且存在多重耐药现象。To investigate the prevalence of the plasmid-mediated quinolone resistance （PMQR） genes in clinical isolates of Enterobacteriaceae from animals in Guangdong province for recent years, 407 strains iso- lated from several farms between 2007 to 2009 were detected for the presence of PMQR determinants by PCR and sequencing . All of the isolates were tested for 15 antimicrobial sensitivity tests by agar dilution method. The results showed that PMQR genes were detected in 92（22.6%） of 407 isolates, including 4 （0.98%） isolated carring qnrA, 20（4.91%） isolates carring qnrB, 66（16.22%） isolates carring qnrS, 7 （1.72~） isolated carring qnrD, 1（0.25%） isolated carring qeDA and 22（5.41%） isolated carring aac（6＇）- Ib-cr gene, respectively. No qnrC positive isolate was detected in this study. Two or more PMQR determi- nants were detected in combination in 27 （6. 63）isolates. Enterobacteriaceae isolated from animals in Guangdong showed high level resistance and also multiple-resistance to Antimicrobial Drugs these years.

关 键 词：PMQR 耐药性 肠杆菌 

分 类 号：S852.612[农业科学—基础兽医学]