酒精Haze改良法测定蔗汁α-葡聚糖含量  

Determination of dextran in sugarcane juice by improved haze method

作　　者：班甲[1] 胡雪梅[1] 徐晓燕[1] 陈骏佳[1] 

机构地区：[1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室,广州510316

出　　处：《食品科技》2012年第9期288-290,共3页Food Science and Technology

基　　金：科技部科研院所技术开发研究专项资金项目(2011EG111248)

摘　　要：为了快速准确测定糖厂蔗汁中α-葡聚糖含量,对传统的酒精Haze法进行改良。利用淀粉酶与α-葡聚糖酶降解蔗汁中的淀粉与α-葡聚糖,以无淀粉与蔗汁α-葡聚糖的蔗汁作为空白制作α-葡聚糖在蔗汁中的工作曲线,测定样品α-葡聚糖含量。试验结果表明:该方法RSD为2.95%,加标回收率在94.2%～96.7%之间。与传统酒精Haze法测定结果相比准确度有了显著提高。该方法克服了其他检测方法的缺点,简化了实验步骤,加快了实验速度,为糖厂实时检测糖汁中的α-葡聚糖含量提供了新的途径。With the purpose of rapid and accurate determination of dextran in sugarcane juice, the traditional haze method was modified. Amylase and dextranase were used to degrade the starch and dextran in sugarcane juice. With starch ＆ dextran-free sugarcane juice as blank control, the dextran content in sugarcane juice were determined. The result proved that the standard deviation was 2.95% and the rate of recovery of the method was between 94.2%-96.7%. The results showed that the accuracy were better than the traditional haze method in the experiment. The method overcomed the disadvantages of other testing methods, simplified the experimental procedures, accelerated the experimental progress, provided a new way of real-time detection of dextran in sugarcane juice in sugar mill.

关 键 词：蔗汁 酒精Haze法 α-葡聚糖 α-葡聚糖酶 

分 类 号：TS247[轻工技术与工程—制糖工程]