皮特兰猪的氟烷基因检测与分子选育  

Detection of halothane gene and molecular breeding in Pietrain pigs

作　　者：谈永松[1] 涂尾龙[1] 胡志刚 王林云[3] 高勤学[4] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海警备区富民农副业基地,上海202181 [3]南京农业大学动物科技学院,江苏南京210095 [4]江苏畜牧兽医职业技术学院,江苏泰州225300

出　　处：《中国兽医学报》2012年第9期1253-1259,共7页Chinese Journal of Veterinary Science

基　　金：上海市科技兴农重点攻关项目(沪农科攻字(2008)第7-1号);上海市农业科技成果转化项目(093919N1500);国家863计划基金资助项目(2008AA101008)

摘　　要：以PCR-RFLPs法为检测方法,针对氟烷基因开展无应激皮特兰猪的分子选育,共检测从2003-2007年若干世代共28批次皮特兰猪。结果显示,在所检测的共994头样本中,具NN基因型的为361头,具Nn基因型的为374头,具nn基因型的为259头。试验结果表明,随着世代选育的进展,群体中N基因和NN基因型的频率逐渐升高,至第17批次时均达100%,后均维持在较高水平,Hard-Weinberg平衡亦呈现平衡-不平衡-平衡的现象,直至此基因最终在群体中失去平衡。试验结果还表明,利用基因技术对家畜进行分子育种是可行的。Molecular breeding of the non-stress Pietrain pigs was carried out for the halothane gene by PCR-RFLPs method,28 batches of several generations Pietrain pigs were detected from 2003 -2007. The results showed that a- mong 994 test pigs there were 361 pigs with NN genotype,374 pigs with Nn genotype,and 259 pigs with nn geno- type. The results implied that, with the progress of generations breeding, the frequency of the N gene and NN geno- type in groups gradually increased and reached to 100% at the 17th batches and then maintained at a high level. Hard-Weinberg equilibrium also presents the phenomenon of balance-imbalance-balance,until this gene was the ulti- mate loss of balance in the population. The results also showed that gene technology was feasible for molecular breeding of livestock.

关 键 词：皮特兰猪 氟烷基因 PCR-RFLPS 分子育种 

分 类 号：S828[农业科学—畜牧学]