鸡Hsc70基因5′侧翼区SNP与耐热性状的相关分析  

Association analysis of 5′flanking region of Hsc70 gene polymorphism and thermo tolerance traits in chicken

作　　者：陈卓宇[1,2] 罗思琳[1,2] 甘建伉[1,2] 张细权[1,2] 罗庆斌[1,2] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东省农业动物基因组学与分子育种重点实验室,广东广州510642

出　　处：《中国兽医学报》2012年第9期1339-1343,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972093)

摘　　要：为研究Hsc70基因作为鸡抗病育种中一种分子标记的可能性,本试验利用克隆测序技术对清远麻鸡、广东温氏矮脚黄鸡、灵山鸡和隐性白羽洛克鸡4个品种共20个个体Hsc70基因的5′侧翼区进行多态性筛查,发现10处变异,包括8个SNPs和2个插入/缺失突变。通过分析,选取C.-521A>C位点,利用PCR-RFLP方法分析了此位点在灵山鸡和隐性白羽洛克鸡群体中的基因型分布,所得分型结果与测定的耐热性状(T3、皮质酮、CD3+、CD4+和CD8+)进行关联分析,结果表明:常温状态下(15℃),在隐性白羽洛克鸡群体中,位点C.-521A>C与CD3+、CD4+和CD8+显著相关(P<0.05),其中AA基因型个体CD3+值显著低于AC基因型个体,而CD4+与CD8+值显著高于AC基因型个体;热应激状态下(35℃),在灵山鸡群体中,位点C.-521A>C与CD8+极显著相关(P<0.01),CC基因型个体CD8+值极显著高于AA和AC基因型。The Hsc70 cytosolic chaperone system facilitates competing pathways of protein folding and degradation. In order to value the effect that the HscTO gene acts as a genetic marker in chicken disease resistance,single nucleotide polymorph isms（SNPs） in the 5＇flanking region of the Hsc70 gene were screened by clone sequencing in 20 individuals from 4 breeds, 10 variations were found,which included 8 SNPs and 2 indel mutations. C.-521A〉C site was genotyped by PCR-RFLP method in Lingshan chicken and white recessive rock. Furthermore, the association of this site with chicken thermo tolerance traits was studied, the result showed that under normal condition（15℃） ,C. -521A〉 C site significantly associated with CD3＋ ,CD4＋ and CD8＋ （P〈0.05） in the white recessive rock,of which value of CD3＋ of AA individual was significantly lower than AC individual,but significantly higher in CD4＋ and CD8＋ value （P〈0.05） ;and under heat stress（35℃） ,C. -521A）C site was significantly associated with CD8＋ （P〈0.01） in the Lingshan chickens,and the CD8＋ value of CC individual was significant higher than the other two genotype individuals.

关 键 词：热应激 Hsc70基因 耐热性状 相关分析 

分 类 号：S852.2[农业科学—基础兽医学]