鹅肝细胞的高纯分离及培养  

Isolation and culture of high-purity goose hepatocytes

作　　者：洪胜辉[1] 张军[1] 邵丹[1] 张宜辉[1] 张蕊[1] 王来娣[1] 龚道清[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国兽医学报》2012年第9期1378-1380,1385,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31072021);江苏省教育厅重大基础研究资助项目(10KJA430054);江苏省高校自然科学基金资助项目(11KJB230005)

摘　　要：采用简单的"胶原酶直接消化法"分离鹅胚胎肝细胞,得到的肝细胞纯度高,活性好;HE染色显示大量双核或多核细胞,细胞具有旺盛增殖分裂能力;肝脏特异性基因白蛋白基因检测显示所分离细胞为成熟肝脏细胞。该法操作简单、高效,是获得鹅原代肝细胞的一种较好的方法,有利于以鹅原代肝细胞为模型的体外研究工作的开展。Using the simple method of collagenase digestion directly＇to isolate goose embryo hepatocytes and can acquire a large high viability and high purity of hepatocytes. HE dyed showed that mostly hepatocytes have two or more nucleus which demonstrate cells have strong proliferation split ability . The specific gene albumin gene test showed that isolated cells are mature hepatocytes. It is a easily and effective method which can get primary goose hepatocyte.

关 键 词：鹅胚胎 鹅原代肝细胞 白蛋白 

分 类 号：S835.2[农业科学—畜牧学]