金纳米花介导的近红外热疗对人喉癌Hep-2细胞增殖的抑制作用  

Inhibition Effect of NIR Photothermal Therapy Mediated by Gold Nanoflowers on Proliferation of Human Laryngocarcinoma Hep-2 Cells

作　　者：宋文植[1] 姜雅萍[1] 纪小会[2] 赵丽丽[2] 尹万忠[3] 杨文胜[2] 

机构地区：[1]吉林大学中日联谊医院,长春130033 [2]吉林大学化学学院 [3]吉林大学第一临床医院,长春130012

出　　处：《高等学校化学学报》2012年第9期1886-1888,共3页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20110708);国家自然科学基金(批准号:50825202)资助

摘　　要：各向异性的金纳米粒子由于其表面等离子共振吸收峰可红移至近红外光区而具有特殊的近红外光热转换性能[1].研究结果表明,纳米棒等各向异性金纳米粒子可将入射的近红外光能转换成热能,从而有效杀伤肿瘤细胞[2].与手术、放化疗等常规治疗相比,金纳米粒子介导的近红外（NIR）热疗具有非侵入及无耐药性等优点,在肿瘤热疗和载药等领域具有广泛应用前景[3,4].传统的各向异性金纳米粒子的制备过程中需引入对生物体有毒的表面活性剂作配体[5,6],限制了其在生物医学领域的应用.Citrate-capped gold nanoflowers （GNFs） prepared by seeding approach were identified to show efficiently photothermal conversion under the irradiation of 808 nm near-infrared laser. MTF [ 3- （4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide ] results demonstrate that the photothermal therapy mediated byGNFs inhibits the proliferation of Hep-2 ceils effectively, and the inhibition effect increases with the increased concentrations of GNFs and power of the laser. The results of apoptosis and necrosis tested by flow cytometry demonstrate that the photothermal ablation play a predominant role in suppressing the proliferation of the Hep-2 ceils. Such citrate-capped GNFs are expected to be a kind of materials potential useful in tumuor NIR photothermal therapy.

关 键 词：金纳米花 柠檬酸钠 近红外热疗 人喉癌Hep-2细胞 热消融 

分 类 号：O614.123[理学—无机化学] O644.18[理学—化学]