胶东地区金黄色葡萄球菌消毒剂抗性基因的研究  

Study on antiseptic resistance genes in Staphylococcus aureus isolates from Jiaodong area

作　　者：李笃军[1] 朱元祺[2] 苏维奇[3] 

机构地区：[1]烟台经济技术开发区医院检验科,山东烟台264006 [2]青岛大学医学院附属医院检验科,山东青岛266003 [3]青岛市市立医院检验科,山东青岛266011

出　　处：《中华医院感染学杂志》2012年第18期3945-3947,共3页Chinese Journal of Nosocomiology

基　　金：山东省科技发展计划项目(2009GG10002057)

摘　　要：目的了解胶东地区临床分离的金黄色葡萄球菌中消毒剂抗性基因携带情况,为临床选择合适消毒剂提供理论依据。方法采用VITEK-2Compact自动微生物分析仪鉴定金黄色葡萄球菌;聚合酶链反应(PCR)检测509株金黄色葡萄球菌中mecA、qacA/B和qacC基因的分布。结果 509株金黄色葡萄球菌mecA、qacA/B和qacC基因的阳性率分别是57.9%、36.9%、8.8%;而在金黄色葡萄球菌mecA基因阳性(耐甲氧西林金黄色葡萄球菌)和阴性(甲氧西林敏感金黄色葡萄球菌)中,qacA/B基因的阳性率分别为50.5%和18.2%,qacC基因的阳性率则分别为9.2%和8.4%。结论胶东地区分离的耐甲氧西林金黄色葡萄球菌中,qacA/B基因的携带率较高,且分布广泛,应引起感染控制部门的重视。OBJECTIVE To update our knowledge of the prevalence and distribution of antiseptic resistance genes in clinical isolates of Staphylococcus aureus so as to provide the theoretical basis for the reasonable selection of disinfectants. METHODS The identification and drug susceptibility testing of the clinical isolates of S. aureus were performed by VITEK2 compact microbial analysis system. Totally 509 clinical S. aureus isolates were screened for the mecA, qacA/B, and qacC genes by PCR. RESULTS The positive rates of mecA, qacA/B, and qacC genes in 509 S. aureus isolates were 57. 9%, 36. 9%, and 8. 8%, respeetively; among the mecA positive methieillin- resistant Staphylococcus aureus strains and mecA negative methicillin-sensitive Staphylococcus aureus, the positive rates of qacA/B were 50. 5% and 18. 2%, respectively, and the positive rates of qacC were 9. 2% and 8.4%, respectively. CONCLUSION These results indicated that qacA/B occurs frequently in S. aureus isolates from Jiaodong area and that the prevalence of antiseptic resistance genes is widespread, thus the infection control departments should attach great importance.

关 键 词：金黄色葡萄球菌 消毒剂抗性基因 qacA/B基因 qacC基因 

分 类 号：R378.11[医药卫生—病原生物学]