碱性清洗剂去除生物膜机制探讨  

Mechanism of alkaline detergent to remove biofilm

作　　者：柴海荣[1] 黎晓晖[2] 杨丽雅[1] 张建辉[1] 陈彦丽[1] 孙称心[1] 郭杰河[1] 丁志平[2] 

机构地区：[1]河北医科大学第二医院供应室,河北石家庄050011 [2]解放军总医院南楼检验科

出　　处：《中华医院感染学杂志》2012年第18期4070-4072,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨碱性清洗剂对去除生物膜机制和效果。方法建立模拟污染物胡克污染物模型和生物膜负载片,使用生物膜清洗剂和酶洗剂在其工作条件下去浸润模拟污染物,肉眼观察胡克污染物模型的污染物去除情况,显微电镜观察生物膜负载片生物膜去除情况,并细菌计数法评价不同清洗剂对生物膜去除效果。结果从胡克污染物模型中可以看出,碱性清洗剂对污染物和生物膜的去除作用机制是减弱物体表面黏合力,同含酶清洗剂作用机制不同,生物膜载片对比试验电镜结果可以看到,碱性清洗剂对生物膜是明显地剥离效果;通过细菌计数法可以看到,生物膜清洗剂碱性清洗剂对生物膜去除效果同阳性对照组NaOH一致基本能达到100.0%,普通含酶清洗剂对生物膜的清除效率只有40.0%,差异有统计学意义。结论碱性清洗剂通过减弱生物膜和物体表面黏合力作用完全清除生物膜,去污能力远远高于普通含酶清洗剂,在模拟试验中相比与含酶清洗剂能够显著清除生物膜。OBJECTIVE To explore the mechanism of alkaline detergent to remove the biofilm and its effect. METHODS The Hucker＇s soil model and the biofilm PCV button model were established, both were immersed in the alkaline and enzymatic detergents to remove the simulated pollutants. The contaminants clearance by the Huskerrs soil model was observed by the visual inspection, and the clearance of biofilm on the PCV button was observed by electron microscope. The effects of different detergents on removing the biofilm were evaluated by the bacterial colony counts method. RESULTS It could concluded from the Hucker＇s soil model that the mechanism of the alkalifie detergent to remove the biofilm is to reduce the adhesive force between biofilm and substrate, which was different from the enzyme-containing detergent. Compared with results of PVC button and the electron microscope, it demonstrated the significant stripping effect by the alkaline detergent. The results of the bacterial colony counts showed that the effect of alkaline detergent was as same as the NaOH of the positive control group on the reducing the biofilm, with the clearance rate almost reaching up to 100%, while the clearance rate of the common enzyme-containing detergent was only 40%, there was an＇ significant difference. CONCLUSION The alkaline detergent can utterly remove the biofilm by reducing the adhesive force between biofilm and substrate,the decontamination effect of which is far better than that of the common enzyme-containing detergent. As compared with the common enzyme-containing detergent, it can significantly remove the biofilm in the simulation test.

关 键 词：生物膜清洗剂 含酶清洗剂 生物膜 胡克污染物模型 

分 类 号：R187[医药卫生—流行病学]