弧菌广谱性外膜蛋白抗原筛选及其单抗的交叉免疫原性研究  

Screening and cross-immunogenicity of broad-spectrum outer membrane protein from Vibrio

作　　者：林海英[1] 马振宁[1] 郑允权[2] 郭养浩[1,2] 唐凤翔[2] 

机构地区：[1]福州大学药物生物技术研究所,福建福州350002 [2]福建省医疗器械与医药技术重点实验室,福建福州350002

出　　处：《福州大学学报（自然科学版）》2012年第4期535-540,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省科技厅重点资助项目(2009N40101;2010N0014);福州大学校基金资助项目(04660022352)

摘　　要：采用生物信息学方法分析几种病原性弧菌OmpK、OmpU和OmpW蛋白种间和种内的同源性,筛选高保守性蛋白OmpW.成功构建具备稳定分泌抗体IgG3的细胞株S5C10,鉴定其对5种弧菌具有特异性交叉免疫反应,而对9种非弧菌无免疫反应.研究结果显示OmpW可作为广谱性疫苗成分,其单抗可特异性检测多元弧菌.Homology of OmpK, OmpU and OmpW proteins from the same and different pathogenic Vibrio species was bioinformaticlly analysed, based on that highly conserved OmpW protein was selected. Hybridoma S5C10 was successfully constructed and stablly secreted IgG3. mAb secreted from S5C10 could specifically cross - reacted with five Vidrio species, but none with nine non - Vibrios. The results showed that OmpW protein could be abroad - spectrum antigen, use of that mAb could specifically detected multi -Vibrios.

关 键 词：弧菌 广谱性 外膜蛋白 单抗 交叉免疫原性 

分 类 号：Q939.91[生物学—微生物学]