人芽囊原虫不同感染途径对免疫抑制小鼠肠黏膜的影响  

Effect of intestinal mucosa for immunosuppressed mice on different infection pathways of Blastocystis hominis

作　　者：王东[1] 薛长贵[2] 

机构地区：[1]河南职工医学院,河南郑州451191 [2]郑州大学医学院

出　　处：《现代预防医学》2012年第18期4813-4815,共3页Modern Preventive Medicine

摘　　要：目的探讨人芽囊原虫(B.hominis)经口和经直肠感染免疫功能低下小鼠后在肠道内寄生和对肠黏膜的损伤情况。方法将昆明小鼠60只应用免疫抑制剂制备为免疫功能低下小鼠后,随机分为6组。其中4组分别经口和直肠感染15×103、20×103剂量的虫体,其他两组为阴性对照组。感染后d 2观察各组小鼠症状并粪便检查感染情况。感染7 d后处死动物,分段将肠内容物涂片检查虫体寄生情况,同时取回盲部肠黏膜观察光镜下的病理结构改变和电镜的超微病理变化。结果经口和直肠感染均显示该虫主要寄生在回盲部和盲肠,主要附着在肠腔和肠黏膜表面。光学和电子显微镜检查显示经口感染小鼠肠黏膜组织病理变化较轻,经直肠感染小鼠肠黏膜病理改变较重,不同感染剂量之间小鼠肠黏膜病理变化无明显差异。正常对照组小鼠肠腔内未见虫体,组织病理检查未见异常改变。结论人芽囊原虫对小鼠肠黏膜损伤程度与感染途径有关,经直肠感染病理变化较重。该虫主要寄生在小鼠回盲部的肠腔和黏膜表面。OBJECTIVE To discuss the parasitism and the injury of intestinal mueosa （B.hominis） through oral and rectum. METHODS 60 Kongndng mouse were randomly immunosuppressed and divided into 6 groups. Four groups were infected with 15x103, 20x103 dose of parasites through oral and rectum, respectively. The remaining two was designated as control group. RESULTS B.hominis mainly parasitized in enteric cavity and on the surface of mucosa of ileocolon and cecum through oral and rectum. The microscope and the electron microscope showed that the histological examination of tissue of caecum revealed light pathological alteration in the group through oral infection, while obvious pathological alteration showed in the intestinal mucosa in the group through rectum infection. There was no obvious distinguish of infection dose. The mouse in control group had no parasites and the structure of mucosa kept normal. CONCLUSION Pathological changes of intestinal mueosa have relations with infection pathway. The condition of pathology become more obvious through rectum infection. B.hominis mainly locates in enteric cavity and on the surface of mucosa of ileocolon.

关 键 词：人芽囊原虫 感染途径 免疫抑制小鼠 肠黏膜 

分 类 号：R38[医药卫生—医学寄生虫学]