培养人眼小梁细胞水通道蛋白1与细胞外基质的相关实验研究  

The effect of aquaporin-1 on extracellular matrix protein expression in cultured human trabecular meshwork cells

作　　者：彭洁[1] 张虹[2] 

机构地区：[1]四川省医学科学院.四川省人民医院眼科,四川成都610072 [2]华中科技大学同济医学院附属同济医院眼科,湖北武汉430030

出　　处：《实用医院临床杂志》2012年第5期34-37,共4页Practical Journal of Clinical Medicine

基　　金：四川省卫生厅科研基金资助项目(编号:100539)

摘　　要：目的探讨人眼小梁细胞上水通道蛋白1(aquaporin-1,AQP1)与细胞外基质中纤维连接蛋白(fibronectin,FN)、层粘连蛋白(laminin,LN)、胶原蛋白Ⅰ(collagen Ⅰ,CAI)及胶原蛋白Ⅳ(collagen Ⅳ,CAIV)的相关关系。方法培养的人眼小梁细胞分别给予地塞米松(dexamethasone,DEX)0.4μg/ml(B组)、DEX0.4μg/ml+AQP1反义寡核苷酸(antisense oligonucle-otides,AS-ODN)0.625μg/ml(C组)、DEX 0.4μg/ml+AQP1AS-ODN 1.25μg/ml(D组)、DEX 0.4μg/ml+AQP1AS-ODN 2.5μg/ml(E组)、DEX 0.4μg/ml+AQP1AS-ODN 5μg/ml(F组)处理,以不加药组为对照组(A组)。5天后,采用免疫组织化学方法和计算机图像分析技术对FN、LN、CAI及CAIV的表达进行检测。结果 0.4μg/ml DEX能刺激培养的人眼小梁细胞FN、LN及CAIV表达明显增强,CAI表达减少,与A组比较差异均有统计学意义(P<0.05)。在C、D、E、F组,AQP1AS-ODN可以降低由0.4μg/ml DEX引起的FN、LN、CAIV表达增强。同样的,增加由0.4μg/ml DEX引起的CAI表达减少。结论人眼小梁细胞AQP1与细胞外基质中FN、LN、CAI及CAIV关系密切。AQP1的正常表达是细胞外基质适量表达的前提。Objective To investigate the correlation between aquaporin-1 （AQP1） and extraeellular matrix in cultured human trabeeular meshwork cells（ HTMC ）. Methods Cultured HTMC were respectively treated with 0.4μg/ml of dexamethasone （ DEX, group B ） or 0.4 μg/ml DEX plus 0. 625 μg/ml （ group C ）, 1.25 p,g/ml （ group D ）, 2.5 μg/ml （ group E ） or 5 μg/ml （ group F） of AQP1 antisense oligonucleotides （AS-ODN）, respectively. Cells without treatment were used as control（ group A）. After 5 days of treatment, the expressions of extracellular matrix proteins such as fibronectin （ FN）, laminin （ LN）, collagen I （CAI） and collagen IV （CAIV） were examined by using immunohistochemical techniques and computer-imaging analysis. Results 0. 4 p,g/ml of DEX increased the expression of FN,LN and CAIV, but decreased the expression of CAI when compared to control. In C, D, E and F groups, AQP1 ASODN revised the effects of DEX on expressions of the extracellular matrix proteins. Conclusions AQPI has an effect on expressions of FN, LN, CAI and CAIV in cultured HTMC. The results suggest that the normal expression of AQP1 may be a key factor for appropriate expression of extracellular matrix proteins.

关 键 词：眼小梁细胞 水通道蛋白1 细胞外基质 纤维连接蛋白 层粘连蛋白 胶原蛋白Ⅰ 胶原蛋白Ⅳ 

分 类 号：R341[医药卫生—基础医学] R322.91