高效液相色谱法测定连花清瘟胶囊中大黄5种成分的含量  

Simultaneous Determination of Five Components From Radixet Rhizoma Rhei in Lianhua Qingwen Capsule by HPLC

作　　者：凌勇[1] 汪艳艳[1] 陈广通[1] 王新杨[1] 

机构地区：[1]南通大学医学院,江苏南通226001

出　　处：《中医学报》2012年第9期1157-1158,共2页Acta Chinese Medicine

基　　金：江苏省高校优势学科建设工程(编号:苏政办发2011-6);南通大学引进人才科研启动基金项目(编号:2011094)

摘　　要：目的:应用高效液相色谱法测定连花清瘟胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种活性成分的含量。方法:流动相采用含有0.1%磷酸的乙腈溶液进行梯度洗脱;流速为1.0 mL.min-1;检测波长为254 nm;柱温30℃。结果:5种活性成分质量浓度与峰面积均呈良好的线性关系,平均回收率分别为99.7%(RSD为1.15%),100.0%(RSD为1.01%),99.8%(RSD为1.37%),99.6%(RSD为0.93%),100.3%(RSD为1.09%)。结论:本测定方法操作简便,结果准确,可靠,重复性好,可用于连花清瘟胶囊的质量控制。Objective：To develop an HPLC method for determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Lian- huaqingwen Capsule. Methods ： Use acetonitrile 0.1% phosphoric acid solution as mobile phase. The flow rate was 1.0 mL·min^-1. The analytes were detected by UV detection at 254 nm, and the eolumn temperature was 30℃. Results ：The average recovery of five compo- nents was 99.7%, 100.0% ,99.8% ,99.6%, 100.3% ,respectively. And the RSD was 1.15% （ n = 5 ）, 1.01% （ n = 5 ）, 1.37% （ n = 5 ） ,0.93% （ n = 5 ）, 1.09% （ n = 5 ）, respectively. Conclusion ： The method was convenient, accurate, and can be used for quantitative determination of the preparation.

关 键 词：连花清瘟胶囊 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 高效液相色谱法 含量测定 

分 类 号：R284.2[医药卫生—中药学]