Annexin V法检测盐胁迫下烟草原生质体的早期凋亡  

Detection of Early Apoptosis of Tobacco Protoplast Under Salt Stress by Annexin V Method

作　　者：张衷华[1] 王化[1] 刘英[1] 祖元刚[1] 

机构地区：[1]森林植物生态学教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2012年第9期93-96,共4页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目(DL12BA05);国家"十一五"科技支撑计划资助项目(2006BAD03A0306)

摘　　要：将膜联蛋白V(Annexin V)法与碘化丙啶(PI)染色法相结合检测烟草(Nicotiana tabacum L.)原生质体的早期凋亡,结果表明:在中性盐胁迫下,能够很好地区分早期凋亡的原生质体和死亡的原生质体。200 mmol.L-1NaCl对烟草原生质体胁迫5 h,凋亡率能达到7.57%;但在200 mmol.L-1NaHCO3碱性盐胁迫下,质膜透性发生了改变,不易区分早期凋亡的原生质体和死亡的原生质体,同时,随着碱性的增加,原生质体破碎率显著升高。碱性的NaHCO3和Na2CO3表现出对细胞膜的破坏作用。An experiment was conducted to test the early apoptosis of tobacco （Nicotiana tabacum L. ） protoplast using Annexin V-fluorescein isothiocyanate/propidium iodide staining. Results showed that, under the stress of neutral salt, early apoptosis and death of protoplasts could be well distinguished. And the apoptosis rate was 7.57% under 200 mmol·L^-1 NaC1 stress for 5 hours. However, under the stress of 200 mmol·L^-1 alkaline salt （NaHCO3 ） , the membrane permeability increased, and the early apoptosis and death of protoplasts couldn＇ t be well distinguished. The broken rate of protoplasts significantly increased with alkaline, which indicated the destructive effect of alkaline salt on membrane.

关 键 词：原生质体 程序性细胞死亡 早期凋亡 盐胁迫 膜联蛋白V法 

分 类 号：Q945.78[生物学—植物学]