正交试验设计法在佛手多糖提取工艺研究中的应用  

Application of the orthogonal experimental design in the research of the extracting technics of fructus citrus sarcodactylis polysaccharide

作　　者：卢梦骁[1] 曹丽英[2] 刘晓茵 

机构地区：[1]同济大学理学院数学系,上海200092 [2]白求恩医科大学制药厂,吉林长春130012 [3]长春医学高等专科学校,吉林长春130031

出　　处：《中国当代医药》2012年第25期160-162,共3页China Modern Medicine

基　　金：吉林省教育厅"十一五"科学技术研究项目(2009548)

摘　　要：目的运用正交试验设计法使佛手多糖提取工艺规范化、科学化。方法以佛手多糖含量为指标,提取次数、提取时间、微波功率、料液比为因素,通过单因素试验确定因素水平,利用正交试验设计对微波法提取佛手多糖的工艺进行优化研究。结果微波法提取佛手多糖的最佳工艺条件为,提取时间:8min,微波提取功率:500W,料液比:1∶20。结论正交试验设计可以为规范化的中药提取工艺研究提供科学依据。Objective To make the extracting technics of fructus citrus sarcodactylis polysaccharide standardized and scien- tific through the orthogonal experimental design. Methods The content of fructus citrus sarcodactylis polysaccharide was used as an indicator, the extracting frequency, extracting time, microwave power, and the proportion of liquids were used as the factors, monofactorial experiment was made to determine the level of actor, and the orthogonal experimental design was used to research on the improved technology for the extraction of fructus citrus sarcodactylis polysaccharide through mi- crowave. Results The optimum conditions for the extraction of fructus citrus sarcodactylis polysaccharide through mi- crowave were summarized as follows： the extracting time was 8 minutes, the extracting power of microwave was 500 W, and the proportion of liquids was 1：20. Conclusion The orthogonal experimental design can provide a scientific basis for the study on standardized extracting technics of traditional chinese medicine.

关 键 词：佛手多糖 正交试验设计 微波 规范化 

分 类 号：R284.2[医药卫生—中药学]