正交试验法优化蔷薇红景天多糖的提取工艺  

Study on Extraction Technology for Polysaccharide from Rhodiola rosea L. by Orthogonal Design

作　　者：邱远金 丁旭[2] 谭勇[2] 张之慧[2] 程喜梅[3] 王绍明[4] 

机构地区：[1]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [2]石河子大学药学院.新疆特种植物药资源省部共建重点实验室,新疆克拉玛依832000 [3]石河子大学化学化工学院,新疆克拉玛依832000 [4]石河子大学生命科学学院,新疆克拉玛依832000

出　　处：《中国药业》2012年第16期55-57,共3页China Pharmaceuticals

基　　金：教育部重点项目;项目编号:208168;兵团科技攻关项目;项目编号:2006GG29

摘　　要：目的优化红景天多糖的最佳提取工艺。方法采用正交试验方法,以多糖含量为考察指标,比较不同水浴浸提温度、超声功率、超声时间以及固液比对提取效果的影响。结果最佳提取工艺为水浴浸提温度90℃,超声功率100%,超声时间30 min,固液比1∶50。结论优选的蔷薇红景天多糖提取工艺合理稳定,可为蔷薇红景天的开发利用提供一定的试验依据。Objective To optimize the extraction conditions of polysaccharide from Rhodiola rosea L..Methods The orthogonal design was used to test the main factors including the temperatue of water bath,ultrasonic power,ultrasonic time and material-liquid ratio with the content of polysaccharide as index.Results The optimum extraction conditions were described as follows：the temperatue of water bath was 90 ℃,ultrasonic power was 100%,ultrasonic time was 30 min and material-liquid ratio was 1 ∶50.Conclusion The optimum extraction conditions is reasonable and stable,which can provide some evidence for development and utilization of Rhodiola rosea L..

关 键 词：蔷薇红景天 多糖 提取工艺 正交试验 紫外分光光度法 

分 类 号：TQ461[化学工程—制药化工] R282.71[医药卫生—中药学]