一测多评法比较不同黄连炮制品中4种生物碱的含量  

Comparison of Contents of Four Alkaloids in Different Processed of Coptis chinensis with Quantitative Analysis Multi-components by Single Marker Method

作　　者：赵君颖[1,2] 汪坤[3] 张振凌[3] 

机构地区：[1]河南省中医院,郑州450002 [2]河南中医学院第二附属医院,郑州450002 [3]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第18期11-13,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2010年中医药行业科研项目(201007010)

摘　　要：目的:比较黄连及其不同炮制品中表小檗碱、黄连碱、巴马汀、小檗碱的含量。方法:应用一测多评法同时测定黄连不同炮制品中4种生物碱的含量。Agilent TC-C18色谱柱(2)(4.6 mm×250 mm,5μm),流动相乙腈-0.05 mol.L-1磷酸二氢钾溶液(50∶50,每100 mL中加十二烷基硫酸钠0.4 g,再以磷酸调节pH 4.0),检测波长345 nm,柱温25℃,流速1 mL.min-1,理论塔板数以盐酸小檗碱计算应大于2 000。结果:黄连经不同方法炮制后表小檗碱、黄连碱、巴马汀、小檗碱的含量均有不同程度的降低。结论:试验建立的含量测定方法快捷、经济,可作为黄连不同炮制品的质量检测方法。Objective： To compare the contents of epiberberine, coptisine, berberine and palmatine in different processed of Coptis chinensis. Method： Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C. chinensis. Chromatographic conditions were： Agilent TC-Cls column （2） （4.6 mm × 250 ram, 5 μm） , mobile phase of acetonitrile-0.05 tool·L^（-1） potassium dihydrogen phosphate （50： 50, plus 0.4 g sodium dodecyl sulfate per 100 mL, phosphate-regulating pH 4.0） , detection wavelength 345 nm, column temperature 25℃, flow rate 1 mL·min^（-1）, number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000. Result： The contents of epiberberine, coptisine, berberine and palmatine in different processed of C. chinensis had different degrees of reduction. Conclusion： Established determination method in this test was rapid and economical, it could be used as quality testing method for different processed of C. chinensis.

关 键 词：一测多评 黄连 炮制品 生物碱 

分 类 号：R283.6[医药卫生—中药学]