舒郁胶囊对原代大鼠海马神经元5-HT3A/3B受体表达的影响  

Effect of Shuyu Capsule on Expression of 5-HT3 Receptor in Primarily Cultured Rat Hippocampus Neurons

作　　者：葛庆芳[1] 姜英凤[1] 张惠云[1] 高杰[2] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,济南250355 [2]山东中医药大学基础医学院,济南250355

出　　处：《中国实验方剂学杂志》2012年第18期136-139,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)资助课题(2011CB505102);国家自然科学基金重点项目(30930110);国家自然科学基金面上项目(81072721);山东省科技发展计划项目(2010GSF10290)

摘　　要：目的:观察抑郁情绪模型组和模型治疗组大鼠血清对原代培养的大鼠海马神经元5-HT3A/3B受体(5-HT3A/3BR)蛋白水平表达的影响,探索复方舒郁胶囊治疗抑郁情绪的微观作用机制。方法:大鼠随机分为5组:正常组、模型组、舒郁胶囊治疗组(0.51 g.kg-1)、柴胡治疗组(0.4 g.kg-1)和氟西汀治疗组(0.002 g.kg-1),采用孤养结合28 d慢性温和刺激(CMS)制备抑郁情绪大鼠模型,治疗组造模的同时灌胃给药,收集各组大鼠血清灭活除菌,干预神经元24 h后利用蛋白印迹技术(Western blot)和细胞免疫荧光技术(immunofluorescence,IF)分别测定神经元胞内5-HT3A/3BR蛋白表达。结果:与正常血清组相比,模型组血清海马神经元5-HT3A/3BR表达水平均显著升高(P<0.01,P<0.05);与模型组比较,治疗组均显著下调5-HT3AR的异常升高状态(P<0.01),但对5-HT3BR表达无明显改变。结论:大鼠海马神经元内5-HT3R表达与抑郁情绪关系密切,5-HT3A/3BR的表达变化可能是导致抑郁情绪的重要病因之一。舒郁胶囊及其君药柴胡含药血清对以上2种蛋白表达的调节趋势与氟西汀一致,对该病症的疗效显著,作用机制可能是调节海马神经元内5-HT3AR的异常表达。Objective：To investigate the expression levels of Serotonin 3A/B receptor（5-HT3A/BR） in primarily cultured rat hippocampus neurons stimulated with serum respectively from depressive rats and different drugs treated depressive rats for exploring mechanisms of Shuyu Capsule.Method：The rats were divided randomly into five groups including normal control,model with depression,treatment groups treated with Shuyu capsule（0.51 g · kg-1）,saikosaponin（0.4 g · kg-1） and fluoxetine（0.002 g · kg-1）,respectively.The depression emotion model was set up by the methods of isolated breed and chronic mild stress for 28 days.Serum of rats in every group was collected,inactivated and sterilized.After serum contained hippocampus neurons treatment in every group respectively for 24 hours,Western blot and immunoflurescence were separately used to detect the expression levels of 5-HT3A and 5-HT3B receptor protein in neurons.Result：The expression of 5-HT3A and 5-HT3B receptor in neurons incubating with serum of rats in model group increased obviously（P〈0.01,P〈0.05）compared with those in the serum from normal control rat treated neurons.Comparing with the neurons treated with model rats serum,the expression of 5-HT3A receptor in neurons incubated with serum of drug treatment rats were significantly improved（P〈0.01）,but the expression of 5-HT3B receptor was no statistical difference.Conclusion：The expression of 5-HT3 receptor in neurons was closely related with depression,and the differential expression of 5-HT3AR and 5-HT3BR may play important roles in depression emotion.Shuyu,saikosaponin and fluoxetine showed the same tendency on these two proteins expression of neurons,indicating that Shuyu capsule and saikosaponin have notable effect on depression therapy by improving the 5-HT3AR protein expression levels in hippocampus neurons

关 键 词：海马神经元 舒郁胶囊 5-HT3受体 

分 类 号：R285.5[医药卫生—中药学]