汉黄芩素对流感病毒感染肺泡巨噬细胞NF-κB核转位及表达的影响及机制  

Effect and Mechanism of Wogonin on NF-κB Nuclear Translocation and Expression in NR8383 Cells Infected by Influenza Virus

作　　者：吴莹[1] 金叶智[1] 张舒[1] 闫玥捷[1] 张津[1] 郝钰[1] 

机构地区：[1]北京中医药大学医学病原系,北京100029

出　　处：《中国实验方剂学杂志》2012年第18期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(30772871);北京中医药大学创新团队"经方现代应用关键科学问题的基础研究"项目(2011-CXD-04)

摘　　要：目的:探讨汉黄芩素对流感病毒感染大鼠肺泡巨噬细胞(NR8383)中核转录因子-κB(NF-κB)核转位、表达的影响及与Toll样受体7(TLR7)介导的髓样分化因子88(MyD88)依赖性信号通路的关系。方法:流感病毒感染NR8383细胞1 h后,加入含汉黄芩素的培养基(终质量浓度0.016 g.L-1),药物作用后6,12,24 h,RT-PCR检测TLR7,MyD88,NF-κB p65mRNA水平;4,6,24 h时,免疫组化法检测NF-κB p65核转位情况,并做半定量分析;24 h时,Western blot检测NF-κB p65表达水平。结果:汉黄芩素降低了流感病毒感染NR8383细胞后TLR7,MyD88,NF-κB p65 mRNA水平(P<0.05,P<0.01,P<0.05),抑制了流感病毒感染后NF-κB p65的核转位及表达(P<0.01)。结论:汉黄芩素通过抑制TLR7介导的MyD88依赖性信号通路,抑制了NF-κB p65的核转位及表达,从而减轻流感感染中的炎症反应。Objective：To investigate the effect of wogonin on the nuclear translocation and expression of nuclear factor-kappa B（NF-κB） in NR8383 cells infected by influenza virus and the relationship with Toll like receptor 7（TLR7） mediated myeloid differentiation factor 88（MyD88）-dependent signaling pathway.Method：After 1 h adsorption of influenza virus,the medium containing 0.016 g · L-1 wogonin was added to NR8383 cells in wells.At 24 h after drug application,reverse transcription polymerase chain reaction（RT-PCR） was used to detect the mRNA level of MyD88,NF-κB p65 and TLR7.At 4,6,24 h after drug application,immunocytochemistry was used to detect nuclear translocation of NF-κB p65 in the cells and perform semi-quantity analysis.At 24 h after wogonin application,Western blot was used to detect the expression of NF-κB p65 protein.Result：Wogonin reduced the mRNA level of TLR7,MyD88,and NF-κB p65（P〈0.05,P〈0.01,P〈0.05）and inhibited the nuclear translocation and expression of NF-κB p65（P〈0.01）.Conclusion：Wogonin inhibited the nuclear translocation and expression of NF-κB by repressing TLR7 mediated MyD88-dependent signaling pathway,and alleviated the inflammatory reaction in influenza virus infection.

关 键 词：汉黄芩素 流感病毒 TOLL样受体7 髓样分化因子88 核转录因子-κBp65 

分 类 号：R285.5[医药卫生—中药学]