双氢青蒿素对AA大鼠外周血T淋巴细胞的调节作用  

Effect of Dihydroartemisinin on Immune-regulation of T-lymphocytes from Peripheral Blood in AA Rats.

作　　者：易剑峰[1] 易少波[1] 雷波[1] 

机构地区：[1]宜春学院美容医学院,江西宜春336000

出　　处：《中国实验方剂学杂志》2012年第18期203-205,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省教育厅科技项目(GJJ08409)

摘　　要：目的:探讨双氢青蒿素对佐剂性关节炎(AA)大鼠外周血T细胞的调节作用。方法:采用佐剂性关节炎动物模型,将体重160～180 g的SD大鼠随机设为6组:正常对照组、模型对照组、甲氨蝶呤对照组(1 mg.kg-1)、双氢青蒿素高、中、低剂量(11.2,5.6,2.8 mg.kg-1)组,造模2周后灌胃给药,连续给药28 d,并采用流式细胞术检测外周血T淋巴细胞亚群、酶联免疫法检测血清白介素-4(IL-4)、干扰素-γ(IFN-γ)的水平。结果:相较于正常组,模型组外周血CD4+T,CD4+T/CD8+T和血清IFN-γ水平显著升高(P<0.01),血清IL-4水平显著降低(P<0.01),CD3+T,CD8+T则无明显变化;高、中剂量双氢青蒿素组CD4+T所占百分比分别是(34.81±3.80)%,(34.92±5.14)%,CD4+T/CD8+T分别是2.21±0.43,2.27±0.48,与模型组相比显著降低(P<0.05,P<0.01);双氢青蒿素高、中、低剂量组血清INF-γ水平分别是(15.90±2.05),(16.27±2.11),(18.15±2.15)ng.L-1,与模型组相比显著降低(P<0.01);IL-4水平分别是(40.21±4.89),(40.04±4.56),(34.81±4.02)ng.L-1,与模型组相比显著升高(P<0.01),其调节作用在2.8～5.6 mg.kg-1范围呈剂量依赖性(P<0.05)。结论:双氢青蒿素能有效改善AA大鼠T细胞功能紊乱状况,为其应用于类风湿关节炎治疗奠定实验基础。Objective：To study the effect of dihydroartemisinin on immune-regulation of T-lymphocytes from peripheral blood in adjuvant-induced arthritis（AA） rats.Method： Sprague Dawley rats with a mean weight of 160-180 g were randomly divided into 6 groups,including normal group,AA model group,methotrexate group（1 mg · kg-1）,dihydroartemisinine high-dose,medium-dose and low-dose group（11.2,5.6,2.8 mg ·kg-1）.The rat adjuvant arthritis model were induced.The drugs were administrated by ig for consecutive 28 days after inducing AA model.T-lymphocyte in peripheral blood was detected by flow cytometry,and the levels of interferon-γ（INF-γ） and interleukin-4（IL-4） in serum were measured by ELISA on AA animal model treated bydihydroartemisinin.Result： Compared with the normal group,the ratio of CD4＋T,CD4＋T/ CD8＋T from peripheral blood in model group and IFN-γ levels in serum were significantly increased（P〈0.01）,and the levels of IL-4 were significantly decreased（P〈0.01）.In high and medium dose group,the percentages of CD4＋T was（34.81±3.80）%,（34.92±5.14）%,the ratio of CD4＋T/ CD8＋T was 2.21±0.43,2.27±0.48,respectively,compared with model group they were significantly decreased（P〈0.05,P〈0.01）.In dihydroartemisinin three dose group,the levels of INF-γ were（15.90±2.05）,（16.27±2.11）,（18.15±2.15） ng·L-1,and the levels of IL-4 were（40.21±4.89）,（40.04±4.56）,（34.81±4.02） ng·L-1,compared with the model group they had significant changes（P〈0.01）.For IL-4,dihydroartemisinin’s effect showed a dose-dependent manner at concentrations between 2.8-5.6 mg · kg-1（P〈0.05）.Conclusion：Dihydroartemisinin could effectively improve the immunity mess in T-lymphocytes from peripheral blood in AA rats,which is useful for curing rheumatoid arthritis.

关 键 词：双氢青蒿素 T淋巴细胞 佐剂性关节炎 

分 类 号：R285.5[医药卫生—中药学]