荧光金逆行标记乳鼠视网膜神经节细胞的实验研究  

Research on retinal ganglion cells of neonatal rats labeled retrogradely with fluorogold

作　　者：李静波[1] 徐尤学 方家华[1] 张祖海[1] 

机构地区：[1]长江大学附属荆州市第一人民医院眼科,荆州434000 [2]湖北省京山县罗店医院眼科

出　　处：《山西医科大学学报》2012年第8期581-583,638,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81000380);湖北省卫生厅青年科技人才基金资助项目(QJX2010-53);湖北省荆州市科技局基金资助项目(2010-2)

摘　　要：目的建立荧光金逆行标记乳鼠视网膜神经节细胞的方法。方法选取出生1 d的SD大鼠乳鼠,腹腔注射水合氯醛麻醉后,剪开头部皮肤,向双侧上丘各注射4%荧光金注射液1μl,缝合皮肤,4 d后处死,提取视网膜,胰酶消化后培养,显微镜下观察被标记的视网膜神经节细胞。结果混合培养的视网膜细胞中,可见被荧光金标记的视网膜神经节细胞,大约占细胞总数的0.34%,视网膜神经节细胞可存活3周。结论荧光金逆行标记乳鼠视网膜神经节细胞是一种可行的方法,可用于视网膜神经节细胞的鉴定。Objective To establish a method to label the retinal ganglion cells of neonatal rats with fluorogold. Methods One-day neonatal Sprague-Dawley rats were anesthetized by intraperitoneal injection with chloral hydrate, and then 1 μl of 4% fluorogold was mi- croinjected bilaterally into the superior colliculi of rats. Four days later, primary mixed retinal cell cultures were established from retinas of five-day neonatal rats. Results Under fluorescence microscope, retinal ganglion cells in mixed cultured retinal cells were success- fully labeled with fluorogold, which accounted for 0.34% of total retinal cells. In addition, retinal ganglion cells survived for three weeks in vitro. Conclusion Retinal ganglion cells of neonatal rats labeled by fluorogold is a feasible approach, and could contribute to the i- dentification of retinal ganglion cells in primary mixed retinal cell cultures .

关 键 词：荧光金 乳鼠 视网膜神经节细胞 

分 类 号：Q813.1[生物学—生物工程]