CD151促进人脐静脉内皮细胞管样结构生成依赖于CD151-整合素α3/α6复合体的形成  

CD151 Promotes Human Umbilical Vein Endothelial Cells Tube Formation Depending on Formation of CD151-integrin α3/α6 Complex

作　　者：费宇杰[1] 左后娟[1] 秦瑾[1] 曾和松[1] 刘正湘[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2012年第4期450-452,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家青年科学基金(No.81000139;No.81000047);高等院校博士学科点专项科研基金新教师基金(No.200804871035)资助项目

摘　　要：目的观察CD151转染人脐静脉内皮细胞(HUVEC)促细胞管样结构形成,及其与整合素的相关性。方法用脂质体转染试剂,将质粒pAAV-CD151及其突变体pAAV-CD151-AAA194-196转染HUVEC,观察转染后细胞在Matrigel胶上管样结构形成情况;免疫共沉淀法检测整合素的表达情况。结果 pAAV-CD151转染组能显著促进HU-VEC管样结构形成,免疫共沉淀能检测到整合素α3、α6的表达;而CD151突变体pAAV-CD151-AAA194-196转染组促HUVEC管样结构形成能力下降,且其免疫共沉淀未能检测到整合素α3、α6的表达。结论 CD151促进HUVEC管样结构形成依赖于CD151-整合素α3/α6复合体的形成。Objective To observe the promotive effects of pAAV-CD151 transfection into human umbilical vein endothelial cells（HUVEC）on tubular morphogenesis,and its correlation with integrins.Methods Using Liposome transfection reagent,plasmid pAAV-CD151 and pAAV-CD151-AAA194-196 mutant were transfected into HUVEC.The tubular morphogenesis on matrigel was observed.Co-immunoprecipitation assay was used to detect the expression of integrins α3 and α6.Results pAAV-CD151 transfection could significantly promote the formation of the tube-like structure of HUVEC,and the expression of integrin α3 and α6 could be detected by using immunoprecipitation.pAAV-CD151-AAA194-196 transfection could not promote the formation of tube-like structure of HUVEC,and the expression of integrins α3 and α6 could not be detected by using immunoprecipitation.Conclusion pAAV-CD151 promoted tubular morphogenesis of HUVEC depending on the formation of CD151-integrin α3/α6 complex.

关 键 词：脐静脉内皮细胞 管样结构形成 CD151 CD151-AAA 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学]