农药西玛津与小牛胸腺DNA的相互作用  

Studies on the interaction of simazine with calf thymus DNA

作　　者：李瑜[1] 张国文[1] 潘军辉[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《南昌大学学报（理科版）》2012年第3期223-227,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金项目(21167013);江西省科技支撑项目(2009BNA09000;2010BSA17400;20112BBF60010);食品科学与技术国家重点实验室目标导向项目(SKLF-MB-201002)

摘　　要：在生理条件(pH 7.4)下,以吖啶橙(AO)为荧光探针,运用荧光光谱、紫外-可见光谱、圆二光谱(CD)和傅里叶转换红外光谱(FT-IR)并结合熔点、粘度及盐效应实验,研究了西玛津与小牛胸腺DNA的相互作用。结果显示,随着溶液中西玛津的浓度增大,DNA-AO复合物的荧光逐渐被猝灭,表明西玛津与AO发生了部分置换作用,而且西玛津的存在使得DNA的熔点和粘度增大,由此推断西玛津与DNA发生嵌插结合。此外,FT-IR光谱分析和盐效应结果表明西玛津与DNA还存在静电结合。计算出的热力学参数表明,疏水作用力是西玛津与DNA结合反应的主要驱动力。The interaction between simazine and calf thymus DNA in a pH 7.4 Tris-HC1 buffer was investi- gated with the use of acridine orange （AO） dye as a fluorescent probe by fluorescence,UV-vis absorption, circular dichroism （CD）, and Fourier transform infrared （FT-IR） spectroscopy, as well as DNA melting studies,viscosity measurements and salt effect. It can be concluded that simazine molecules could interca- late into the base pairs of DNA as evidenced by slgnificant fluoresce ce quenching of the DNA-AO complex with the binding of simazine to DNA by substituting for AO in the DNA-AO complex,and increase in melt- ing temperature and relative viscosity of DNA. Furthermore,the FT-IR spectroscopy and salt effect demon- strated that there exists electrostatic attraction between simazine and DNA simultaneously. The thermody- namic parameters suggested that the binding of simazine to DNA was driven mainly by hydrophobic inter- actions.

关 键 词：西玛津 小牛胸腺DNA 相互作用 光谱法 

分 类 号：O657.3[理学—分析化学]