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机构地区:[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]保山学院资源环境学院,云南保山678000
出 处:《西南农业学报》2012年第4期1205-1210,共6页Southwest China Journal of Agricultural Sciences
基 金:国家自然科学基金(31000712)
摘 要:为研究不同方法破除休眠处理对小麦新种子SOD活性的影响,将新收获的3个品系小麦种子(KQ11、KQ3和KQ27)分别用低温(2、4、6、8℃分别处理3、6、9、12 d)、H2O2(浓度为0%、0.31%、0.63%、0.95%,在18、20、22℃下分别处理8、16、24 h)、GA3(浓度为0、0.025‰、0.050‰、0.075‰、0.100‰,18、20、22℃下分别处理8、16、24 h)进行破除休眠处理,并测定分析种子SOD活性。结果表明,当以低温处理时,4℃处理SOD活性最高,2℃处理最低;以H2O2处理时SOD活性受处理时间影响较大;以GA3处理时处理浓度、处理温度、处理时间和试验材料都对SOD活性有极显著影响。用H2O2和GA3处理小麦种子破除休眠时,处理时间不能过长,以免对种子造成伤害。To study the different methods for breaking dormancy on SOD activity of wheat seeds,the three lines freshly harvested wheat seeds,KQ11,KQ3 and KQ27 were treated with low temperature(2,4,6,8 ℃ and 3,6,9,12 d,respectively),H2O2(0 %,0.31 %,0.63 %,0.95 %,and 18,20,22 ℃ for 8,16,24 h,respectively),GA3(0,0.025‰,0.050‰,0.075‰,0.100‰,and 18,20,22 ℃ for 8,16,24 h,respectively)to breakdormancy,and the SOD activity of treated seeds were analyzed.The results showed that SOD activity was the highest when treated at 4 ℃,but was the lowest when treated at 2 ℃ under low temperature;SOD activity were affected significantly by treatment time under H2O2;and SOD activity were affected significantly by treatment concentration,temperature,time and test material under GA3.The treatment time should not be too long to prevent wheat seeds injury when treated with H2O2 and GA3.
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