4个野生灵芝菌株的ITS序列分析  

Analysis of ITS Sequences in 4 Wild Ganoderma lucidum Strains

作　　者：贾定洪[1] 王波[1] 彭卫红[1] 黄忠乾[1] 谭伟[1] 甘炳成[1] 郑林用[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066

出　　处：《西南农业学报》2012年第4期1414-1416,共3页Southwest China Journal of Agricultural Sciences

基　　金：科技部国际合作资助项目(2010DFA32080);四川省科技厅国合项目(2011HH0025);国家食用菌产业技术体系栽培与设施研究室岗位(毛木耳和药用菌栽培);国家食用菌产业技术体系成都综合试验站;四川食用菌产业技术体系创新团队;四川省财政基因工程资助项目

摘　　要：以4个野生灵芝菌株为研究材料,以6个外源菌株为参考菌株,采用MEGA4软件对ITS序列进行NJ法聚类分析,构建系统发育树。结果显示4个灵芝菌株ITS序列与另外6个参考菌株对齐序列长度在357～369 bp之间,G/C含量变化为43.4%～57.7%;德昌灵芝1#、德昌灵芝4#和通江灵芝亲缘关系最近,遗传距离为0,德昌灵芝3#与它们的遗传距离为0.0029;与4个野生灵芝菌株亲缘关系由近到远分别是韩芝、红芝、灵芝(中国科学院微生物研究所)、黑灵芝、血芝、云芝;结合4个野生菌株的ITS序列分析结果和形态特征,4个菌株被鉴定为灵芝(Ganoderma lucidum)种类的不同株系。With the bio-software of MEGA4,the ITS sequences of 4 wild Ganoderma lucidum strains and their 6 reference strains were studied by using NJ method to construct the phylogenetic tree.The results showed that the ITS lengths of their strains ranged from 357 to 369 bp,and G/C contents from 43.4 % to 57.7 %.There were the closest relationships among Dechang lingzhi1#,Dechang lingzhi4# and Tongjiang lingzhi,and their strains＇genetic distance was merely 0,while there was a genetic distance of 0.0029 between Dechang lingzhi3# and them.Strains,from the close genetic relationships to the farther relationships with the 4 wild Ganoderma lucidum strains,were in order of Hanzhi,Hongzhi,Lingzhi（The Institute of Microbiology,Chinese Academy of Sciences）,Heilingzhi,Xiezhi,Yunzhi,respectively.Comparative study of ITS sequences and morphic characteristics indicated that 4 wild strains were subject to different strains of Ganoderma lucidum.

关 键 词：灵芝 ITS 系统发育 

分 类 号：S567.41[农业科学—中草药栽培]