矮牡丹花粉形态观察与萌发特性研究  

Morphological Observation and Pollen Viability of Dwarf Peony

作　　者：贾文庆[1] 尤扬[1] 刘会超[1] 刘美玲[1] 

机构地区：[1]河南科技学院园林学院,河南新乡453003

出　　处：《西北林学院学报》2012年第5期76-79,183,共5页Journal of Northwest Forestry University

基　　金：河南省重点科技攻关项目"牡丹组培工厂化育苗关键技术研究"(112102110021)

摘　　要：以矮牡丹花粉为材料,研究花粉的形态及不同培养基对矮牡丹花粉萌发及花粉管伸长的影响。结果表明,花粉萌发率与长球形花粉占花粉总数的百分率一致;适合矮牡丹花粉萌发的培养基为9%蔗糖+0.004 5%硼酸;低浓度(1×10-5～1×10-3 mol/L)的Ca2+促进了花粉管的伸长,高浓度(1×10-2～1×10-1 mol/L)的Ca2+抑制了花粉管的伸长,适合花粉管伸长的Ca2+浓度为1×10-3 mol/L;低浓度(0.1×10-3 mol/L)的Mg2+促进了花粉管的伸长,高浓度(0.2×10-3～1.8×10-3 mol/L)的Mg2+抑制了花粉管的伸长,适合花粉管伸长的浓度为Mg2+0.1×10-3 mol/L。Pollen morphology of dwarf peony and the effects of different cultural media on pollen germina- tion and pollen tube elongation were evaluated. The results indicated that the pollen germinating percent- age was consistent with the proportion of prolate pollens. Culture media with different concentrations of sucrose and boric had good effects on the pollen germination. The pollen germinating percentage reached the highest after incubated on the media supplemented with 9% sucrose and 0. 045 % boric acid. Low con- centrations （1×10-5 --1×10-3 mol/L） of Ca2＋ had good effect on pollen tube elongation, while high con- centrations （1×10-2-1×10-1 mol/L） inhibited them. The most suitable concentration of Ca2＋ to pro- mote pollen tube elongation was 1×10-3 mol/L in the culture medium. Low concentration （0. 1×10 3 mol/L） Mg2＋ had good effect on pollen tube elongation, while high concentration （0.2×10.3 --1.8×10.3 mol/L） Mg2＋ exhibited inhibited effects. The most suitable concentration of Mg2＋ to promote pollen tube elongation was 0.1×10.3 mol/L in the culture medium.

关 键 词：矮牡丹 形态观察 花粉管伸长 CA2+ Mg2+ 

分 类 号：S685.11[农业科学—观赏园艺]