九龙江口海岸带沉积物甲烷相关古菌群落结构及多样性研究  

Stratified Communities and Diversity of Methanogen in Sediments of Jiulong River Estuary

作　　者：许可[1] 陈金全[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]云南大学工程技术研究院,云南昆明650091

出　　处：《安徽农业科学》2012年第26期12752-12753,12760,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(51168046)

摘　　要：[目的]针对河口环境的甲烷产生相关微生物展开调查,了解其多样性及群落结构组成、变化。[方法]采用克隆文库技术,分析九龙江河口沉积物样品,调查甲烷产生相关微生物的多样性及分布情况。[结果]九龙江沉积物含有清晰的甲烷硫酸盐过渡区。克隆文库分析结果显示主要是Methanosaeta,Methanomicrobiales和Methanomsarcinales/ANME。Methanomsarcinales/ANME是主要类群(41%),且以甲烷氧化菌ANME-2a为主。Methanosaeta和Methanomicrobiales分别占27%和28%。[结论]在九龙江河口沉积物中,乙酸利用型和氢气利用型甲烷产生是主要的甲烷产生途径。[ Objective] To investigate the diversity and distribution of methanogen in the sediments of Jinlong River estuary. [ Method ] Based on clone library analysis, the diversity and distribution of methanogen communities in sediments of Jiulong River estuary were investigated. [ Result] The majority of obtained sequences in clone library were assigned mainly to Methanosaeta, Methanomicrobiales and Methanomsarcinales/ANME. The sequences of Methanomsarcinales/ANME were the most dominant group （41% of total sequences） and composed largely of ANME-2a. In ad- dition, Methanosaeta and Methanomicrobiales accounted for 27% and 28% of all sequences. [ Conclusion ] Acetoclastic and hydrogenotrophic methanogenesis were the main methanogenic pathway in Jiulong River estuary sediments.

关 键 词：产甲烷生物 甲烷生成 厌氧甲烷氧化. 

分 类 号：S181.3[农业科学—农业基础科学]