硒化壳寡糖对成骨细胞的作用  

Effects of Chitooligosaccharide-selenium on Osteoblasts

作　　者：刘宇桐[1] 金黎明[1] 徐鑫[1] 撒楠[1] 鲍丽[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《安徽农业科学》2012年第26期12778-12779,共2页Journal of Anhui Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金资助项目(DC110318;DC120101053);大连民族学院"太阳鸟"项目

摘　　要：[目的]研究硒化壳寡糖对体外培养的成骨细胞MC3T3-E1的作用。[方法]将硒化壳寡糖分别以10、100、500、1 000、2 000μg/ml的浓度加入培养基中,通过MTT测定法观察其对体外培养的成骨细胞的作用。[结果]在试验设置的5个浓度梯度下,硒化壳寡糖均能促进成骨细胞增殖,且效果优于壳寡糖,其中500μg/ml为最适浓度。[结论]其机理还有待于进一步探索。[ Objective] Effects of chitooligosaceharide-selenium（COS-Se） on osteoblasts MC3T3-E1 in vitro were studied. [ Method] COS-Se was added into culture medium at concentrations of 10, 100, 500, 1 000, 2 000 μg/ml, respectively and then MTT method was used for measurement. [ Result ] The results showed that COS-Se with concentrations of the five could all promote proliferation of osteohlasts, which was better than COS. The optimal concentration was 500 μg/ml. [ Conclusion] The mechanism requires further exploration.

关 键 词：成骨细胞 硒化壳寡糖 MTT测定法 

分 类 号：S188[农业科学—农业基础科学]