鸡血藤DNA提取方法的研究  

Study on the Extraction Methods of Genomic DNA from Caulis spatholobi

作　　者：姜建萍[1] 田辉[1] 崔健[1] 封毅[1] 

机构地区：[1]广西中医药大学药学院,广西南宁530001

出　　处：《安徽农业科学》2012年第26期12782-12783,共2页Journal of Anhui Agricultural Sciences

基　　金：广西壮族自治区卫生厅中医药科技专项(GZKZ10-003);广西自然科学基金面上项目(2012GXNSFAA053117)

摘　　要：[目的]筛选一种适合鸡血藤DNA的提取方法。[方法]以鸡血藤嫩叶为材料,分别采用液氮-CTAB法、石英砂-CTAB法以及石英砂-SDS法提取基因组DNA,利用琼脂糖凝胶电泳和紫外分光光度计检测提取的DNA样品,对提取方法进行比较筛选。[结果]电泳检测中,石英砂-CTAB法所提DNA条带亮度最高,质量较好。紫外检测中,石英砂-CTAB法提取的DNA A260/A280值大于1.8,比其他两种方法提取的DNA蛋白质污染小,其提取的DNA纯度和得率均最高,液氮-CTAB法提取DNA得率居中,石英砂-SDS法提取DNA得率较低。[结论]该研究结果表明石英砂比液氮更能简便快速地将叶片研碎,CTAB提取缓冲液比SDS提取缓冲液可更有效地去除杂质,使细胞裂解、DNA析出。因此,石英砂-CTAB法更适合鸡血藤DNA的提取。[ Objective] To find out a suitable method for extracting genome DNA from Caulis spatholobi. [ Method] Genome DNA was extracted from the young leaves of C. spatholobi by the method of liquid nitrogen-CTAB, quartz sand-CTAB and quartz sand-SDS. The extracted genome DNA was then determined through UV spectrophotometer and agarose gel electrophoresis. And the best extraction method was selected. [ Result ] According to the elcctrophoresis detection, the brightness of DNA strap extracted by the method of quartz sand-CTAB was the highest, and the quality was much better. According to the UV detection, with the quartz sand-CTAB method, A260/A280 of DNA was greater than 1.8, the protein＇ s contamination was less than other two methods, and the purity and yield were the highest. The yield of DNA extracted by liquid nitrogen- CTAB method was medium and the yield of DNA extracted by quartz sand-SDS method was lower. [ Conclusion] The results indicated that the leaf blade was levigated more conveniently and fleetly by quartz sand than liquid nitrogen. The cell was spirted and the DNA was separated out more efficiently by CTAB buffer than SDS buffer. Therefore, quartz sand-CTAB method was much fitter for the extraction of genome DNA from Caulis spatholobi.

关 键 词：鸡血藤 DNA 提取 

分 类 号：S567[农业科学—中草药栽培]