从不同材料中提取马DNA方法的比较研究  

Comparative Study of the Horse DNA Extraction Methods from Different Materials

作　　者：马晓婷[1] 刘芳[1] 陈亚丽[1] 姜鑫[1] 马军[1] 罗娇娇[1] 褚仲春[1] 邵勇钢[1] 

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052

出　　处：《安徽农业科学》2012年第26期12787-12788,共2页Journal of Anhui Agricultural Sciences

基　　金：新疆农业大学自治区重点产业紧缺人才专业大学生创新项目资助(jqrcp32010014)

摘　　要：[目的]通过对5种从不同材料中提取马DNA的方法进行比较研究,寻找最适的提取方法。[方法]采用酚仿抽提法、蛋白酶K直接消化法、酸碱裂解法分别从血液、毛发材料中提取马DNA,并通过PCR扩增对提取产物进行检测。[结果]通过蛋白酶K直接消化法、酸碱裂解法可从微量血液和毛发中提取到马DNA,均可达到采用酚仿抽提法从血液中提取马DNA的效果,并能满足后续分子生物学试验的要求。[结论]通过比较分析,该研究中以酸碱裂解法最佳。[Objective] To compare five kinds of horse DNA extraction methods from different materials,so as to find the best one. [Method] phenol and chloroform method,proteinase K direct digestion method and acid-alkali lysis method were performed to extract DNA from the horse blood and hair respectively,and PCR amplification was used to detect the extracted product. [ Result] Proteinase K direct digestion method and acid-alkali lysis method can be used to extract DNA from the horse trace blood and hair and able to meet the requirements of the subsequent molecular biological analysis. [ Conclusion] Through comparative analysis,the acid-alkali lysis method was the best.

关 键 词：马 DNA提取 材料 方法 

分 类 号：S821[农业科学—畜牧学]