广东不同产地南板蓝根药材中腺苷含量的测定  

Comparison of the Content of Adenosine Contained in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from Different Production Places in Guangdong Province

作　　者：李世杰[1] 陈奕龙[1] 张丹雁[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405

出　　处：《安徽农业科学》2012年第26期12852-12854,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省科技计划项目(2010B030700067)

摘　　要：[目的]测定广东不同产地南板蓝根药材中有效成分腺苷的含量。[方法]建立南板蓝根药材中腺苷的HPLC含量测定方法;色谱条件为:ZORBAX Extend-C18色谱柱(4.6 mm×250 mm,5μm);流动相为无水甲醇-水(7∶93,V/V);检测波长为260 nm;流速为1.0ml/min;柱温为30℃。[结果]在4.65～232.6 ng/ml范围内,腺苷浓度与峰面积有良好的线性关系,加样回收率为100.3%,RSD为2.2%(n=6);广东从化市样品中的腺苷含量最高,2种野生品种含量最低。[结论]该方法操作简便、准确快速、重现性好,可有效控制南板蓝根药材的质量。[ Objective ] To make the use of high performance liquid chromatographic method （HPLC） to determine the content of active ingredient adenosine in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from different production places in Guangdong Province. [ Method] A HPLC method was established for determining the content of adenosine. To be specific, HPLC analysis was performed on a chromatographic column ZORBAXExtend-C18 （4.6 mm×250 mm,5μm） with methanol-water（ V/V, 7：93 ） as the mobile phase, detection wavelength at 260 nm, flow rate at 1.0 ml/min and column temperature at 30 ℃. [ Result] Adenosine had good linear relationship between concentration and peak area from 4.65 to 232.6 ng, the recovery rate was 100.3% , RSD = 2. 2% （ n = 6） ; the sample from Conghua in Guangdong Province had the highest content, while the two wild species had the lowest of that. [ Conclusion] The method is simple, accurate, rapid and reproducible. It ean complete the existing quality standard of RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE compound feed additives, and make the control of quality efficiently.

关 键 词：南板蓝根 腺苷 HPLC 

分 类 号：S567[农业科学—中草药栽培]