副鸡嗜血杆菌的分离与鉴定  

Isolation and Identification of Haemophilus paragallinarum

作　　者：张洪辉[1,2] 王汉清[1] 王蓓[1] 李宁[1] 曹守林[1] 倪欣涛[1] 王桂军[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省濉溪县动物卫生监督所,安徽濉溪235100

出　　处：《安徽农业科学》2012年第26期12913-12914,共2页Journal of Anhui Agricultural Sciences

基　　金：安徽省战略性科技攻关项目(11010302119);安徽省年度重点科研项目(11070303024)

摘　　要：[目的]对1例鸡传染性鼻炎疑似病例进行病原菌的分离与鉴定。[方法]从安徽省某鸡场发生的以脸面肿胀、流泪和产蛋下降为临床特征的病鸡上呼吸道分泌物中分离细菌,并进行染色、镜检、菌落形态观察,并利用"卫星现象"和PCR等方法进行鉴定。[结果]成功分离到1株副鸡嗜血杆菌,经鉴定为C型副鸡嗜血杆菌。动物回归试验表明,该分离株能引起典型的鸡传染性鼻炎,对鸡具有较强的致病力。[结论]该研究可为鸡传染性鼻炎的预防与控制提供依据。[ Objective ] To isolate and identify a suspected case of infectious coryza in chickens. [ Method ] The bacteria were isolated from the upper respiratory tract excretions in the chickens, which had the clinical symptoms of swelling faces, tears and declining egg production. The bacteria were dyed and observed under microscopy, and then identified with satellitosis and PCR methods. [ Result] A strain of Haemophilus paragallinarum was successfully isolated, and then identified to be C types. According to the animal regression test, tile isolated strain may induce the strongly infectious coryza in chickens. [ Conclusion] The study provides basis for the prevention and control of chicken infectious coryza.

关 键 词：副鸡嗜血杆菌 分离 鉴定 PCR 传染性鼻炎 

分 类 号：S855.1[农业科学—临床兽医学]